猪伪狂犬病-传染性胃肠炎二联高免血清最佳免疫程序及保存条件的初步筛选

发布时间：2018-03-27 07:52

  本文选题：猪伪狂犬病　切入点：猪传染性胃肠炎　出处：《黑龙江畜牧兽医》2017年01期

【摘要】：为了获得高效价的猪伪狂犬病-传染性胃肠炎二联高免血清,试验采用冻融法制备猪免疫血清,ELISA阻断法、血凝试验及血凝抑制试验分别检测各组猪伪狂犬病病毒、猪传染性胃肠炎病毒血清抗体效价,比较试验组及空白对照组两种病原的血清抗体效价。将两种病原抗体效价最高组血清分别置不同温度保存不同时间检测其抗体效价,探讨最适保存条件。结果表明:S6组的两种病原血清抗体效价最高,猪伪犬病病毒血清OD630值为0.352、猪传染胃肠炎病毒血清抗体效价为1∶64;试验组与空白对照组之间、S6组与其他试验组之间抗体效价差异显著。说明S6组为此次探讨的猪伪狂犬病-传染性胃肠炎二联高免血清的最佳免疫程序,通过30 d的基础免疫及10~15 d的强化免疫能获得较高效价的猪伪狂犬病病毒、猪传染性胃肠炎病毒血清抗体。-20℃和4℃分别适合猪伪狂犬病-传染性胃肠炎二联高免血清抗体液的长期和短期保存。
[Abstract]:In order to obtain the high titer of porcine pseudorabies and infectious gastroenteritis high immune serum, the porcine immune serum was prepared by freezing and thawing method. The hemagglutination test and hemagglutination inhibition test were used to detect pseudorabies virus in each group. The titer of serum antibody against infectious gastroenteritis virus was compared between the test group and the blank control group. The results showed that the antibody titers of the two pathogens were the highest in the two groups. The serum OD630 value of porcine pseudoviruses was 0.352, and the titer of serum antibody to porcine gastroenteritis virus was 1: 64. There were significant differences in antibody titers between S6 group and control group and other experimental groups, which indicated that S6 group was the pig pseudo virus in this study. The best immunization procedure for rabies and infectious gastroenteritis. The higher titer of porcine pseudorabies virus could be obtained by 30 days basic immunization and 1015 days intensive immunization. Serum antibody of porcine infectious gastroenteritis virus. -20 鈩，

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