日粮中共轭亚油酸对小鼠红细胞数量影响的研究

发布时间：2018-03-29 04:17

本文选题：共轭亚油酸　切入点：红细胞数量　出处：《河南农业大学》2015年硕士论文

【摘要】：共轭亚油酸(CLA)是一种常用的食品添加剂,但是动物试验表明日粮中添加CLA会造成明显的代谢障碍,如脂肪肝、高血糖等。同时CLA是一种不饱和脂肪酸,可对红细胞膜脂质双层造成一定的影响。已有研究表明,给老年大鼠饲喂添加CLA的饲料可使红细胞膜总n-6不饱和脂肪酸含量显著降低,n-6/n-3比值会下降,而这种变化可能对红细胞膜流动性、渗透脆性及溶血速率产生不良影响。但是,饲喂添加CLA的饲料对红细胞数量的影响尚未见报道。CLA可导致小鼠代谢障碍,这在本实验室先前的研究中已有报道。本论文在CLA导致小鼠代谢障碍的情况下,对红细胞数量的改变及其可能的原因进行了研究。首先,给39日龄昆明白雄性小鼠饲喂含1.5%共轭亚油酸的饲料(CLA组,n=8),以1.5%的亚油酸为对照组(LA组,n=8),CLA组小鼠表现出脂肪肝和脂肪组织减少为特征的代谢障碍。然后,检测两组小鼠RBC(红细胞计数)、HGB(血红蛋白浓度)、HCT(红细胞压积)、MCV(平均红细胞体积),结果显示,与LA组相比,CLA组小鼠RBC减少了48.96%,HCT减少了46.49%,MCV升高了7.82%,差异均显著(P0.01),CLA组小鼠HGB有降低的趋势(差异不显著)。连续检测发现,在饲喂的第47,61,86天,红细胞数量较LA组减少,差异显著(P0.05)。CLA组小鼠的血液网织红细胞数量是LA组的两倍多(P0.01)。此结果提示CLA组小鼠骨髓造血功能代偿性增强。本论文又对日粮中CLA降低小鼠红细胞数量的机制进行了探讨。首先,我们检测了红细胞膜的渗透脆性,结果发现CLA组小鼠与LA组小鼠之间并无显著变化(P0.05)。随后,Q-PCR法检测小鼠肾脏EPO基因表达量以及亚铁嗪比色法检测血清铁含量,发现CLA组小鼠与LA组间并无显著差异,但是ELASA试剂盒检测VB12、叶酸含量发现,CLA组小鼠的血浆VB12含量较LA组小鼠减少了24.72%(P0.05),血浆中叶酸含量则减少了23.15%(P0.01)。并且,血常规检测结果显示,与LA组相比,CLA组小鼠的单核细胞比例(MONO%)升高了41.77倍(P0.05)。上述结果提示,CLA组小鼠红细胞数量减少的原因可能是红细胞合成原料(VB12和叶酸)不足,或单核细胞吞噬红细胞的作用加强,或者两者都是。结论:日粮中CLA使小鼠红细胞数量减少;血浆中VB12和叶酸含量降低以及MONO%升高可能是红细胞数量减少的原因;CLA组网织红细胞数量增多代表了骨髓造血代偿性增强。
[Abstract]:Conjugated linoleic acid (CLA) is a common food additive, but animal experiments have shown that dietary CLA can cause obvious metabolic disorders, such as fatty liver, hyperglycemia, etc. CLA is also a kind of unsaturated fatty acid. It has been shown that the content of total n-6 unsaturated fatty acids in erythrocyte membrane decreased significantly in aged rats fed with CLA, and the ratio of n-6 / n-3 decreased significantly. This change may have a negative effect on erythrocyte membrane fluidity, osmotic fragility and hemolysis rate. However, the effect of diet supplemented with CLA on erythrocyte number has not been reported. This has been reported in previous studies in our laboratory. In this study, changes in erythrocyte count and their possible causes were studied in mice with metabolic disorders caused by CLA. Kunming white mice were fed with 1.5% conjugated linoleic acid (CLA) diet for 39 days, and 1.5% linoleic acid was used as control group. The mice in the LA group showed metabolic disorders characterized by fatty liver and adipose tissue reduction. The RBC (erythrocyte count) HGB( hemoglobin concentration) HCT (mean erythrocyte volume) in the two groups were detected. Compared with LA group, RBC decreased by 48.96% and 46.49%, and increased 7.82%. The difference was significant (P 0.01) compared with LA group (the difference was not significant). Continuous test showed that the number of red blood cells was lower than that of LA group on the 47th day, 6186 days after feeding, the number of RBC was lower than that of LA group. The number of reticulocyte in the CLA group was more than twice that in the LA group. The results suggested that the bone marrow hematopoietic function of the CLA group was compensatory. The mechanism of CLA decreasing the erythrocyte count in the diet was also discussed in this paper. The osmotic fragility of erythrocyte membrane was detected. The results showed that there was no significant change between CLA group and LA group. Subsequently, the expression of EPO gene in kidney of mice was detected by Q-PCR and the serum iron content was detected by ferrizine colorimetry. It was found that there was no significant difference between CLA group and LA group, but VB12 was detected by ELASA kit. Compared with LA group, the content of plasma VB12 decreased 24.72% (P 0.05), and the content of plasma folic acid decreased 23.15% (P 0.01). Compared with LA group, monocyte ratio in CLA group was 41.77 times higher than that in LA group (P 0.05). These results suggested that the decrease of erythrocyte number in CLA group might be due to the deficiency of VB12 and folic acid, or the enhancement of monocyte phagocytosis. Conclusion: CLA in diet can reduce the number of red blood cells in mice. The decrease of plasma levels of VB12 and folic acid and the increase of mono% may be the reason for the decrease of erythrocyte number. The increase of reticulocyte represents the enhancement of hematopoiesis compensability in bone marrow.
【学位授予单位】：河南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S816

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