短花针茅荒漠草原土壤微生物群落组成及结构

发布时间：2018-03-29 10:48

本文选题：细菌多样性　切入点：真菌多样性　出处：《生态学报》2017年15期

【摘要】：为详细了解内蒙古短花针茅荒漠草原生态系统中土壤微生物群落组成与结构。对其土壤中微生物的总DNA提取后,采用高通量测序技术对土壤中细菌的16Sr DNA和真菌的ITS基因进行了序列测定,分析了短花针茅荒漠草原土壤中微生物群落结构特征。结果表明,共获得细菌OTUs13711个,真菌OTUs 5929个。物种分类显示,细菌种类隶属于29门57纲111目191科485属,其中优势类群为Gammaproteobacteria和Thermoleophilia,它们的相对丰度分别为32.68%和26.83%。真菌隶属于4门16纲45目78科105属,优势类群为Ascomycota和Basidiomycota,它们的相对丰度分别为35.76%和25.90%。土壤中细菌群落的多样性和丰富度均高于真菌。
[Abstract]:In order to understand the composition and structure of soil microbial community in the desert steppe of Stipa breviflora in Inner Mongolia, the total DNA was extracted from the soil. The high throughput sequencing technique was used to sequence the 16Sr DNA gene of bacteria and the ITS gene of fungi in soil, and the structure of microbial community in the soil of Stipa breviflora desert steppe was analyzed. The results showed that a total of OTUs13711 bacteria were obtained. The species taxonomy of fungi OTUs 5929.The species classification showed that the bacteria belong to 29 phyla, 57 classes, 111 orders, 191 families and 485 genera, among which the dominant groups were Gammaproteobacteria and Thermoleophilia, their relative abundance were 32.68% and 26.833%, respectively. The fungi belonged to 105 genera, 45 orders, 78 families and 4 phyla, 16 classes, 45 orders, 78 families, respectively. The dominant groups were Ascomycota and Basidiomycota, their relative abundance were 35.76% and 25.90 respectively. The diversity and richness of bacterial communities in soil were higher than that of fungi.
【作者单位】：内蒙古农业大学生态环境学院;内蒙古师范大学生命科学与技术学院;天津师范大学生命科学学院;
【基金】：国家自然科学基金面上资助项目(31270502) 内蒙古自治区自然科学基金资助项目(2015MS0371) 内蒙古师范大学基金资助项目(2015YBXM013)
【分类号】：S812.2

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