微囊化大肠杆菌口服灭活疫苗对大鼠免疫效果的研究

发布时间：2018-03-29 20:57

本文选题：大肠杆菌　切入点：口服疫苗　出处：《畜牧兽医学报》2017年02期

【摘要】：不同血清型的致病性大肠杆菌是引起幼畜腹泻的主要病因之一。笔者拟对采用微囊包被技术制备的羊大肠杆菌灭活口服疫苗对大鼠的免疫效果进行评价。将制备的羊大肠杆菌蜂胶佐剂灭活苗作为芯材,天然高分子材料海藻酸钠为壁材,采用喷雾干燥法制备微囊化口服疫苗。微囊化口服疫苗的含菌量为7.52×1011菌·g-1。100只成年健康大鼠随机分为对照组、注射组和微囊包被口服疫苗组(再分为基础剂量、10倍基础剂量和20倍基础剂量三组),免疫后采用微量凝集试验和本室建立的间接ELISA方法检测免疫血清抗体效价并对细胞和黏膜免疫进行检测。微量凝集试验检测结果显示,免疫后7d各试验组即可产生抗体,免疫后28d时抗体效价达到高峰,随后开始下降。间接ELISA方法检测结果显示,免疫后14d各试验组特异抗体转阳,并持续到28d。口服组的抗体效价均显著高于对照组(P0.05),而与注射组无差异(P0.05),且各口服剂量组之间差异不显著(P0.05)。免疫后35d口服试验组特异抗体转为阴性,而注射组可维持到35d;T淋巴细胞转化结果显示,免疫7d后,三个口服剂量组的SI值均显著高于对照组和注射组(P0.05)。免疫后口服各剂量组的肠黏膜分泌型免疫球蛋白A(sIgA)含量均高于同期的对照组和注射组,21d后sIgA含量达到高峰。微囊包被口服疫苗能够产生与注射组同样的体液免疫,同时还可以刺激大鼠机体产生较好的细胞免疫和黏膜免疫。
[Abstract]:The pathogenic Escherichia coli of different serotypes is one of the main causes of diarrhea in young animals. The author intends to evaluate the immune effect of oral inactivated oral vaccine of Escherichia coli from sheep prepared by microencapsulation technique in rats. Of sheep Escherichia coli propolis adjuvant inactivated vaccine as core material, Sodium alginate, a natural macromolecule material, was used to prepare oral microencapsulated vaccine by spray drying method. The microencapsulated oral vaccine contained 7.52 脳 1011 bacteria and 1.100 healthy adult rats were randomly divided into control group. The injection group and the microencapsulated oral vaccine group were divided into three groups: the basic dose was 10 times and the basic dose was 20 times. The antibody titers of immune serum were detected by microagglutination test and indirect ELISA method established in our laboratory after immunization. Cell and mucosal immunity were detected. Microagglutination test showed that, The antibody titers reached the peak at 28 days after immunization, and then began to decrease. Indirect ELISA assay showed that the specific antibodies of each test group turned positive 14 days after immunization. The titer of antibody in oral group was significantly higher than that in control group, but there was no difference between oral group and injection group, and there was no significant difference between each oral dose group. The specific antibody in oral test group turned negative 35 days after immunization. The results of T lymphocyte transformation in the injection group were maintained until 35 days after immunization for 7 days. The SI values of the three oral dose groups were significantly higher than those of the control group and the injection group (P 0.05). The levels of secretory immunoglobulin A (IgA) in the intestinal mucosa of the three groups were higher than those in the control group and the injection group 21 days after immunization. The microencapsulated oral vaccine produces the same humoral immunity as the injection group. At the same time, it can stimulate the rat body to produce better cellular immunity and mucosal immunity.
【作者单位】：新疆农业大学动物医学学院;阿拉山口出入境检验检疫局;新疆合普联科检测技术研究院;
【基金】：新疆维吾尔自治区科技厅高技术项目(200911112) 新疆农业大学研究生科研创新项目(XIAUGRI2015013) 重点研发计划项目(2016YFD0500508)
【分类号】：S859.7

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