不同蛋白质水平日粮对梅花鹿IGF-1和GH基因表达的影响

发布时间：2018-03-29 21:30

  本文选题：梅花鹿　切入点：蛋白质水平　出处：《基因组学与应用生物学》2017年11期

【摘要】：本试验旨在探讨不同蛋白质水平日粮对梅花鹿胰岛素样生长因子-1(IGF-1)基因和生长激素GH基因m RNA表达量的影响,为科学配置梅花鹿饲料及鹿茸生长提供理论基础。选取27头4岁梅花鹿公鹿为试验对象,随机分成3组,分别饲喂不同蛋白质水平的日粮(Ⅰ:20%,Ⅱ:24%,Ⅲ:28%)。采用SYBR Green Real-time PCR方法检测不同蛋白质水平日粮对梅花鹿鹿茸组织IGF-1基因和GH基因的表达情况。结果表明:不同蛋白质水平日粮显著影响鹿茸组织IGF-1和GH基因的表达风度,随着蛋白质水平的增加,IGF-1基因在鹿茸组织上的表达量降低,Ⅰ组鹿茸表达量最高,显著高于Ⅱ组、Ⅲ组(p0.05),GH基因在Ⅲ组鹿茸表达量最高,显著高于Ⅰ组、Ⅱ组(p0.05)。
[Abstract]:The purpose of this study was to investigate the effects of different protein levels on the expression of IGF-1 gene and GH gene m RNA in sika deer. To provide the theoretical basis for the scientific allocation of sika deer feed and antler growth, 27 four-year-old sika deer male deer were selected as experimental objects, and were randomly divided into 3 groups. SYBR Green Real-time PCR method was used to detect the expression of IGF-1 gene and GH gene in deer antler tissues of sika deer with different protein levels (I: 20, 鈪，

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