基因工程鲎素对金黄色葡萄球菌小鼠感染模型治疗效果

发布时间：2018-03-30 01:20

本文选题：小鼠　切入点：基因工程鲎素　出处：《中国兽医学报》2017年04期

【摘要】：基因工程鲎素体外抑菌活性良好,为进一步评价其在体内抑菌活性和治疗效果,本研究建立了金黄色葡萄球菌小鼠肺部和腹腔感染模型,探讨了基因工程鲎素对2种感染模型小鼠的治疗效果。比较基因工程鲎素治疗前后,不同模型小鼠的存活率、血液以及组织中的细菌载量变化;比较治疗前后小鼠肺部感染动物模型的病理学变化。结果表明,与对照组相比,基因工程鲎素治疗后肺部感染模型小鼠存活率升高40%,腹腔感染模型小鼠存活率升高60%,治疗后腹腔感染小鼠血液中的细菌载量下降10~4倍;病理学分析结果表明,基因工程鲎素治疗后小鼠肺脏的炎症反应较轻,肺部组织结构被破坏程度较轻。综上所述基因工程鲎素对金黄色葡萄球菌感染小鼠的体内治疗效果明显,显著提高了小鼠存活率,抑制了细菌的扩散,减轻了病理炎症变化,初步表明基因工程鲎素具有良好的抗感染药物研发前景。
[Abstract]:In order to further evaluate its antibacterial activity and therapeutic effect in vivo, a model of pulmonary and abdominal infection of Staphylococcus aureus mice was established in this study. The therapeutic effect of genetic engineering Limulus lysin on two infected mice was studied. The survival rate and bacterial load in blood and tissue of different model mice were compared before and after treatment with genetic engineering Limulus lysin. The pathological changes of pulmonary infection in mice before and after treatment were compared. The results showed that, compared with the control group, The survival rate of mice with pulmonary infection was increased by 40%, the survival rate of mice with intraperitoneal infection was increased by 60%, and the bacterial load in blood of mice with intraperitoneal infection was decreased by 10 ~ 4 times after treatment. The inflammatory reaction in the lungs of mice treated with genetic engineering TAL was lighter and the lung tissue structure was destroyed less. In conclusion, the therapeutic effect of GE-TAL on the mice infected with Staphylococcus aureus in vivo was obvious. The survival rate of mice was significantly increased, the proliferation of bacteria was inhibited, and the pathological inflammatory changes were alleviated, which indicated that genetic engineering TAL had a good prospect for the development of anti-infective drugs.
【作者单位】：吉林大学动物医学学院;吉林大学动物科学学院;
【基金】：吉林省科技发展计划重大科技招标专项资助项目(20150203008NY)
【分类号】：S859.79

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