1株PRRSV类NADC30毒株的分离鉴定及序列分析

发布时间：2018-03-30 08:02

本文选题：猪繁殖与呼吸综合征病毒　切入点：分离鉴定　出处：《河南农业科学》2017年09期

【摘要】：为了解猪繁殖与呼吸综合征病毒(PRRSV)流行株的变异情况,将PRRSV阳性病料处理后接种Marc-145细胞,成功分离到1株PRRSV毒株,将其命名为HENZXC-4,并对其全基因组序列进行测定和分析。结果表明,HENZXC-4为未发生重组的类NADC30毒株,其NSP2蛋白存在131个氨基酸不连续缺失,且其GP5蛋白存在1个氨基酸插入。
[Abstract]:In order to understand the variation of porcine reproductive and respiratory syndrome virus (PRRSVV) epidemic strain, Marc-145 cells were inoculated with PRRSV positive venereal material, and one PRRSV strain was isolated successfully. It was named HENZXC-4 and its whole genome sequence was determined and analyzed. The results showed that HENZXC-4 was a NADC30 like virus strain without recombination, its NSP2 protein had 131 amino acid discontinuities and its GP5 protein had one amino acid insertion.
【作者单位】：河南农业大学牧医工程学院;
【基金】：河南省高校科技创新团队与支持计划资助项目(14IRTSHN015)
【分类号】：S852.651

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