副猪嗜血杆菌PCR检测方法的建立与应用

发布时间：2018-03-30 08:50

  本文选题：副猪嗜血杆菌　切入点：PCR　出处：《畜牧与兽医》2017年02期

【摘要】：为了快速检测临床上副猪嗜血杆菌(Haemophilus parasuis,Hps)感染,根据Gen Bank中Hps的16S rRNA基因保守区序列,设计1对特异性引物,利用PCR方法检测Hps,并进行特异性、敏感性及临床检测。结果显示:PCR反应选择58℃为最适退火温度,0.5μL(20μmol/L)为最适引物量,模板浓度为7.2×10~(-6)μg/mL时即可扩增出814 bp特异性目的片段,与细菌分离结果一致。结果表明:该方法敏感、快速、特异,可用于临床上Hps感染的快速检测。
[Abstract]:In order to detect Haemophilus parasuis Hpss infection in clinic quickly, according to the 16s rRNA gene conserved region sequence of Hps in Gen Bank, a pair of specific primers were designed and detected by PCR method. Sensitivity and clinical test. The results showed that 814 BP specific target fragment could be amplified when the optimal annealing temperature was 58 鈩，

本文编号：1685191