禽呼肠孤病毒感染后Toll样受体在SPF鸡不同器官中的表达变化

发布时间：2018-03-30 21:21

本文选题：禽呼肠孤病毒　切入点：鸡Toll样受体　出处：《中国兽医科学》2017年02期

【摘要】：为了探明Toll样受体(TLRs)在禽呼肠孤病毒(ARV)感染致病过程中的作用,本研究考察ARV感染鸡的不同器官中TLRs在不同感染时间点的动态表达变化。本研究将ARV S1133毒株经足垫注射,接种7日龄SPF鸡,感染后第1、3、5、7和14天采集鸡脾、腔上囊、胸腺、肝和心脏等器官组织,利用荧光定量PCR方法检测TLR3、TLR5、TLR7、TLR15和TLR21在这些器官中的m RNA表达水平变化,分析ARV感染对鸡不同器官中上述TLRs m RNA表达量的影响。结果表明,ARV可诱导TLR3、TLR5、TLR7、TLR15和TLR21在不同器官中的表达,并且表达变化也不同。在免疫器官中以TLR3和TLR7的表达升高为主,在心脏和肝中以TLR7和TLR15的表达为主。这些结果提示,TLR3、TLR5、TLR7、TLR15和TLR21在ARV感染致病过程中发挥不同的作用,本研究结果为进一步阐明禽呼肠孤病毒的致病机理奠定了基础。
[Abstract]:To investigate the role of Toll like receptor (TLRs) in the pathogenesis of avian reovirus (ARV) infection, the dynamic expression of TLRs in different organs of chicken infected with ARV was investigated in this study.In this study, ARV S1133 strain was injected with foot pad and inoculated into SPF chickens of 7 days old. The spleen, bursa, thymus, liver and heart of chickens were collected on the 7th and 14th days after infection.The changes of m RNA expression of TLR3, TLR5, TLR7, TLR15 and TLR21 in these organs were detected by fluorescence quantitative PCR method, and the effect of ARV infection on the expression of TLRs m RNA in different organs of chicken was analyzed.The results showed that the expression of TLR3, TLR5, TLR7, TLR15 and TLR21 in different organs could be induced by ARV, and the expression changes were also different.The expression of TLR3 and TLR7 was increased mainly in immune organs and TLR7 and TLR15 in heart and liver.These results suggest that TLR3, TLR5, TLR7, TLR15 and TLR21 play different roles in the pathogenesis of ARV infection. These results provide a basis for further elucidating the pathogenesis of avian reovirus.
【作者单位】：广西壮族自治区兽医研究所广西兽医生物技术重点实验室;广西大学动物科学技术学院;
【基金】：广西自然科学基金项目(2014GXNSFCA118006) 广西水产畜牧兽医局科技项目(桂渔牧科201452003) 国家自然科学基金项目(31660715) 国家“万人计划”领军人才专项(2016-37) 广西兽医生物技术室开放基金项目(14-045-31-A-2)
【分类号】：S852.65

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