石林县仔猪腹泻病原分析

发布时间：2018-03-31 12:06

本文选题：仔猪　切入点：腹泻　出处：《云南农业大学》2016年硕士论文

【摘要】：仔猪腹泻是生猪产业发展中经常发生而又难以彻底解决的问题,每年因仔猪腹泻造成的经济损失非常巨大,为了解石林县致仔猪腹泻的细菌性和病毒性的病因,为规模化养殖场和农村散养户预防及治疗仔猪腹泻提供依据。本研究对所采集的9个规模化养殖场腹泻仔猪样品185份和28户散养户样品48份进行了主要病毒性及细菌性病原的检测。通过对233份样品进行细菌分离、镜检、生化反应等鉴定。结果显示,233份样品中大肠杆菌的阳性数为233份,阳性率为100%;233份样品中沙门氏菌阳性数为41份,阳性率为17.60%;其中,规模养殖场阳性34份,阳性率18.38%;散养户阳性7份,阳性率14.58%。提示,大肠杆菌是致石林县仔猪腹泻的主要细菌性病原。对分离的233株大肠杆菌及41株沙门氏菌进行了药物敏感性试验,其中大肠杆菌对氨苄青霉素的耐药率高达83.3%,其次是红霉素(66.7%)。沙门氏菌对红霉素的耐药性高达100%,对氯霉素、氨苄青霉素和庆大霉素耐药性均超过了80%。采用RT-PCR、PCR方法对233份样品的仔猪腹泻主要病毒性病原进行了检测。结果为:圆环病毒2型检出率最高,共有111份样品圆环病毒2型阳性,阳性率47.64%,其中,规模养殖场检出84份,阳性率45.41%,散养户27份,阳性率56.25%。流行性腹泻病毒阳性13份,阳性率5.78%;其中,规模养殖场8份,阳性率4.32%;散养户5份,阳性率10.42%。轮状病毒检测出6份,阳性率2.58%,其中:规模养殖场1份,阳性率0.54%;散养户5份,阳性率10.42%。伪狂犬病毒与传染性胃肠炎病毒均未检出。以上结果表明:致石林县仔猪腹泻的病原较为复杂,且存在多病原混合感染的现象。其中,大肠杆菌和圆环病毒2型是主要的引起仔猪腹泻的细菌性和病毒性病原。本研究结果为规模化养殖场和农村散养户预防和治疗仔猪腹泻提供了依据。
[Abstract]:Piglet diarrhea is a common and difficult problem in the development of pig industry. The economic loss caused by piglet diarrhea is very huge every year. In order to understand the cause of bacterial and viral diarrhea caused by piglet diarrhea in Shilin County, In this study, 185 samples of diarrhea piglets and 48 samples of piglets collected from 9 large scale farms and 28 scattered households were tested for the prevention and treatment of piglet diarrhea. Detection of bacterial pathogens. Isolation of 233 samples, The results showed that the positive number of Escherichia coli in 233 samples was 233, the positive rate of Salmonella in 100 233 samples was 41, the positive rate was 17.600.Among them, 34 were positive in large-scale farms. The positive rate was 18.38%; the positive rate was 14.58% in scattered households. It was suggested that Escherichia coli was the main bacterial pathogen causing diarrhea in piglets in Shilin County. The drug sensitivity tests of 233 strains of Escherichia coli and 41 strains of salmonella were carried out. Among them, the resistance rate of Escherichia coli to ampicillin was as high as 83.3%, followed by erythromycin 66.7%. The resistance of Salmonella to erythromycin was as high as 100%, and to chloramphenicol. The drug resistance of ampicillin and gentamicin exceeded 80%. RT-PCR was used to detect the main viral pathogens of diarrhea in 233 piglets. The results showed that the detection rate of circovirus type 2 was the highest, and 111 samples were positive for circovirus type 2. The positive rate was 47.64, of which 84 were detected in large-scale farms, and the positive rates were 45.41, 27 in scattered households and 56.25.The positive rate of epidemic diarrhea virus was 13, and the positive rate was 5.78; among them, 8 were in large-scale farms, with a positive rate of 4.32 and 5 in retail households. The positive rate of rotavirus was 10.42. Six rotavirus samples were detected, and the positive rate was 2.58. The positive rate was 10.42%. Neither pseudorabies virus nor infectious gastroenteritis virus was detected. The above results show that the pathogen of diarrhea in piglets in Shilin County is more complicated, and there is a mixed infection of multiple pathogens. Escherichia coli and circovirus type 2 are the main bacterial and viral pathogens causing piglet diarrhea. The results provide a basis for the prevention and treatment of piglet diarrhea in large-scale breeding farms and rural scattered farmers.
【学位授予单位】：云南农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S858.28

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