DNA甲基化酶抑制剂对鸡脂肪发育相关基因表达的影响

发布时间：2018-04-01 15:15

本文选题：DNA甲基化　切入点：脂肪发育　出处：《黑龙江畜牧兽医》2017年09期

【摘要】：为了研究DNA甲基化在调控脂肪生长发育中的作用,试验采用甲基化酶抑制剂(5-azadc)处理鸡原代前脂肪细胞,采用Real-time RT-PCR方法分析鸡脂肪生长发育相关基因(PPAR长、C/EBPB、HOPX、KLF7、A-FABP)的表达变化。结果表明:甲基化酶抑制剂处理均不同程度地提高了PPAR抑、C/EBPB、HOPX、KLF7、A-FABP基因的表达。农业部鸡遗传育种重点实验室前期研究结果也发现,高、低脂肉鸡腹部脂肪组织的DNA甲基化水平存在差异,提示DNA甲基化参与鸡脂肪组织生长发育的调控。
[Abstract]:In order to study the DNA methylation in the regulation of fat growth in the role of test by methylation enzyme inhibitor (5-azadc) treatment of primary chicken preadipocytes, using Real-time RT-PCR method to analyze the growth and development of chicken fat related genes (PPAR, C/EBPB, HOPX, KLF7, A-FABP). The results show that the expression of methyltransferase the inhibitor treatments increased the inhibition of PPAR, C/EBPB, HOPX, KLF7, A-FABP gene expression. The Ministry of agriculture chicken genetic breeding Key Laboratory preliminary results also found that high levels of DNA methylation in low fat broiler abdominal fat tissue differences, suggesting that regulation of DNA methylation in chicken adipose tissue growth.

【作者单位】：东北农业大学动物科学技术学院;黑龙江八一农垦大学生命科学技术学院;
【基金】：“973”国家重点基础研究发展计划项目(2009CB941604) “863”国家高技术研究发展计划项目(2011AA100301)
【分类号】：S831

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