鸭坦布苏病毒间接ELISA抗体检测试剂盒的初步应用

发布时间：2018-04-03 08:00

本文选题：鸭坦布苏病毒　切入点：间接ELISA　出处：《中国家禽》2017年19期

【摘要】：为了开发鸭坦布苏病毒间接ELISA抗体检测试剂盒,研究利用灭活纯化的鸭坦布苏病毒(DTMUV)FX2010株作为包被抗原,由瑞普(保定)生物药业有限公司连续制备了5批次鸭坦布苏病毒间接ELISA抗体检测试剂盒中试产品。该试剂盒对禽流感病毒、鸭瘟病毒、鸭细小病毒、Ⅰ型鸭肝炎病毒、鸭呼肠孤病毒阳性血清和5份SPF阴性质控血清进行的检测均无交叉反应,表明具有较好的特异性。批内和批间重复性试验的变异系数为1.5%~4.2%和2.2%~5.3%,表明产品具有很好的稳定性。应用该试剂盒及中和试验对300份疑似DTMUV阳性血清样品进行检测,结果显示:二者阳性样品符合率为90.12%,阴性样品符合率为93.6%,总符合率为92.7%。研究结果表明,该试剂盒具有操作简便、敏感性高和特异性强的特点,可以为鸭坦布苏病毒病的快速诊断及其流行病学调查提供有力的工具。
[Abstract]:In order to develop a kit for the detection of indirect ELISA antibody against tambusuvirus, the inactivated and purified strain of duck tambusuvirus (DTMUV) FX2010 was used as the coated antigen.Five batches of indirect ELISA antibody test kit for duck tabusu virus were prepared by Ruip (Baoding) Biopharmaceutical Co., Ltd.The kit had no cross reaction for avian influenza virus, duck plague virus, duck parvovirus, duck hepatitis I virus, duck reovirus positive serum and 5 SPF negative quality control sera.The coefficients of variation of intra-and inter-assay reproducibility tests were 1.5% and 2.2% respectively, which indicated that the products had good stability.This kit and neutralization test were used to detect 300 suspected DTMUV positive serum samples. The results showed that the coincidence rate of the two positive samples was 90.12, the coincidence rate of negative samples was 93.66.The total coincidence rate was 92.7%.The results showed that the kit had the characteristics of simple operation, high sensitivity and strong specificity, and could provide a powerful tool for the rapid diagnosis and epidemiological investigation of TBV.
【作者单位】：瑞普(保定)生物药业有限公司;中国农业科学院上海兽医研究所;
【基金】：河北省省级省校科技合作开发资金支持项目“坦布苏病毒ELISA抗体检测试剂盒的开发及产业化” 国家重点研发计划项目(2016YFD0500800) 国家自然科学基金青年科学基金项目(31702237) 上海市自然科学基金项目(17ZR1437400) 中央级公益性科研院所基本业务费项目(2017JB09)
【分类号】：S858.32

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