猪源肺炎克雷伯菌的分离鉴定及其耐药性分析

发布时间：2018-04-04 19:18

  本文选题：肺炎克雷伯菌　切入点：整合子　出处：《中国兽医科学》2017年12期

【摘要】：通过菌落形态观察、生化试验及16S rDNA基因序列测定对分离菌株进行鉴定,共获得7株猪源肺炎克雷伯菌,将其命名为Kp1~Kp7。致病性试验结果表明,7株分离菌株对小鼠均具有较强的致病性。药敏试验结果显示,分离菌株的耐药表型差异性较大,对强力霉素、大观霉素及阿米卡星的耐药性较强。对分离菌株整合子的检测表明,仅能从Kp1检测到消毒剂-磺胺基因(qac EΔ1-sul1)、整合酶基因(IntⅠ)以及基因盒。本研究为肺炎克雷伯菌引起的疾病选择合理的抗菌药物提供了一定的科学依据,并为进一步研究其耐药机制奠定了基础。
[Abstract]:Seven strains of Klebsiella pneumoniae were identified by colony morphology, biochemical test and 16s rDNA gene sequencing.The results of pathogenicity test showed that all of the 7 isolated strains had strong pathogenicity to mice.The results of drug sensitivity test showed that the resistant phenotypes of the isolates were different, and the resistance to doxycycline, spectinomycin and amikacin was stronger.The detection of integron in isolated strains showed that only disinfectant sulfanilamide gene (QAC E 螖 1-sul1), integrase gene (Int 鈪，

本文编号：1711318