肠炎沙门菌SDBL-1分离株的生物学特性及传播途径探究

发布时间：2018-04-04 19:12

本文选题：肠炎沙门菌　切入点：传播途径　出处：《畜牧兽医学报》2017年07期

【摘要】：为研究肠炎沙门菌(S.enteritidis)的生物学特性和传播途径,选择1株来自于AA肉鸡的肠炎沙门菌SDBL-1分离株,探究其半数致死量、生长特性、药敏试验、毒力因子;将SDBL-1分离株定量至7.09×109CFU·mL-1,感染组灌服0.5mL,同时对照组和空气传播组灌服等量的生理盐水,在灌服后不同时间测定空气传播组、感染组和对照组血液常规、淋巴细胞亚群指标,运用细菌分离的方法分析鸡在灌服后0~20d排毒规律,同时对空气传播组口腔拭子、泄殖腔拭子、羽毛囊以及剖检脏器等分离与鉴定沙门菌,测定环境(饲料、饮水、蚊蝇等)中沙门菌;应用平板凝集方法测定空气传播组抗体变化。结果显示:1)血液指标方面,空气传播组、感染组淋巴细胞比率下降,粒细胞比率、粒细胞数量以及中间细胞比率上升,同时淋巴细胞亚群CD4+、CD8+以及CD4+/CD8+比例出现下降;2)脏器指数方面,空气传播组肝指数显著增大(P0.05),胸腺指数显著减小(P0.05);3)感染情况,空气传播组发生感染,感染鸡通过粪便间歇性排毒,其口腔拭子、羽毛囊、泄殖腔拭子和部分脏器分离到沙门菌,其中沙门菌在食道的分离率最高,空气传播组在感染后第4天初次检测到沙门菌抗体,在感染后第14天血清均呈现抗体阳性;4)环境沙门菌检出情况,水线及饲料中分离到沙门菌,但羽毛和蚊蝇中没有分离到沙门菌。可见SDBL-1分离株能通过空气传播并引起鸡的感染,导致机体炎症反应,造成免疫功能紊乱和器官不同程度损伤;感染鸡通过粪便、口腔、泄殖腔间歇性排毒,环境拭子(如料槽中的饲料、水线)可分离到沙门菌,本研究探究了肠炎沙门菌的生物学特性和不同传播途径,为控制、预防沙门菌的流行和传播提供借鉴。
[Abstract]:In order to study the biological characteristics and transmission pathway of Salmonella enteritidistis, a SDBL-1 isolate from AA broiler was selected to investigate its lethal dose, growth characteristics, drug sensitivity test and virulence factor.The SDBL-1 isolates were quantified to 7.09 脳 109CFU mL -1. The infected group was given 0.5 mL, and the control group and the air transmission group were administered with the same amount of normal saline. The blood routine and lymphocyte subsets were measured at different time points in the air transmission group, the infection group and the control group.The method of bacterial isolation was used to analyze the excretion regularity of chickens at 0 ~ 20 days after administration. At the same time, the oral swabs, cloacal swabs, hairy follicles and organs were isolated and identified, and the environment (feed, drinking water) was also determined in the air transmission group, such as oral swabs, cloacal swabs, hairy follicles and dissecting organs.Salmonella in mosquitoes and flies etc. The antibody changes of airborne transmission group were determined by plate agglutination method.The results showed that in the blood index, the lymphocyte ratio decreased, the granulocyte ratio, the granulocyte number and the intermediate cell ratio increased in the air-borne group and the infected group.At the same time, the ratio of lymphocyte subsets, CD4, CD8 and CD4 / CD8 decreased. In the aspect of organ index, the liver index of airborne transmission group increased significantly, and the thymus index decreased significantly.Salmonella was isolated from oral swabs, feather follicles, cloacal swabs and some organs of infected chickens through fecal intermittent detoxification. The isolation rate of salmonella in esophagus was the highest.In the air transmission group, Salmonella antibodies were detected for the first time on the 4th day after infection, and on the 14th day after infection, antibodies were detected in the sera of the air transmission group. Salmonella was isolated in the water line and feed.But no salmonella was isolated from feathers and mosquitoes.It can be seen that the SDBL-1 isolate can spread through the air and cause chicken infection, leading to inflammatory reaction of the body, resulting in immune dysfunction and organ damage in varying degrees; the infected chicken intermittently detoxifies through feces, mouth and cloacal cavity.Salmonella can be isolated from environmental swabs (such as feed in feed tank, waterline). This study explored the biological characteristics and different transmission routes of Salmonella enteritis, which provided reference for controlling and preventing the spread of Salmonella enteritidis.
【作者单位】：山东农业大学;
【基金】：国家十三五重点项目(2016YFD0501608) 现代农业产业技术体系建设专项资金(nycytx-41-k14)
【分类号】：S852.61

【相似文献】