静原鸡ELOVL2和ELOVL5基因表达的组织特异性研究

发布时间：2018-04-08 20:47

本文选题：静原鸡　切入点：ELOVL　出处：《浙江农业学报》2017年08期

【摘要】：采用实时荧光定量PCR(qRT-PCR)技术对宁夏地方优良品种静原鸡的肝脏、肌肉、心脏、睾丸、卵巢、腹脂6个组织中ELOVL2和ELOVL5基因的表达量进行了测定,并通过SAS8.2软件进行分析统计。结果表明,ELOVL2和ELOVL5基因在静原鸡6个不同组织中均有表达。其中ELOVL2基因在肝脏中的相对表达量最高,为71.52±0.41,显著高于其他组织(P0.05);其次是卵巢、睾丸、腹脂、心脏及肌肉,在另外5个组织中表达量虽有差异,但均差异不显著(P0.05)。ELOVL5基因在静原鸡不同组织中的相对表达量依次为:肝脏腹脂卵巢睾丸心脏肌肉,同样在肝脏中的表达量最高,为110.94±0.02,显著高于其他组织(P0.05),在腹脂中的表达量显著高于卵巢、睾丸、心脏和肌肉(P0.05)。另外在心脏和肌肉中的表达量均差异不显著(P0.05)。对ELOVL2和ELOVL5基因分别在不同组织中的表达差异进行分析比较,发现两个基因在腹脂中的表达量差异显著(P0.05),ELOVL5基因在腹脂中的表达量较高。研究结果说明,ELOVL2和ELOVL5基因在静原鸡6种组织中的表达存在差异,并以肝脏组织最为明显,这为地方品种的开发与利用、种质资源遗传评定及地方品种分子育种技术平台的构建奠定了一定的理论基础。
[Abstract]:The results showed that both ELOVL2 and ELOVL5 genes were expressed in 6 different tissues of Chicken.The relative expression of ELOVL2 gene in liver was the highest (71.52 卤0.41), which was significantly higher than that in other tissues (P 0.05), followed by ovary, testis, abdominal fat, heart and muscle.However, the relative expression of P0.05N. ELOVL5 gene in different tissues of Chicken was in the order of liver, abdominal fat, ovary, testis, heart muscle, and the highest expression in liver.The expression of P0.05 in abdominal fat was significantly higher than that in ovary, testis, heart and muscle.In addition, there was no significant difference in the expression of P0.05 in the heart and muscle.The differences of expression of ELOVL2 and ELOVL5 genes in different tissues were analyzed and compared. It was found that the expression of ELOVL2 and ELOVL5 genes in abdominal fat was significantly different, and the expression of ELOVL5 gene in abdominal fat was higher than that in abdominal fat.The results showed that there were differences between EloVL2 and ELOVL5 gene expression in six tissues of Jingyuan chicken, and the liver tissue was the most obvious, which was the development and utilization of local breeds.Genetic evaluation of germplasm resources and the construction of molecular breeding platform for local varieties lay a certain theoretical foundation.
【作者单位】：宁夏大学农学院;
【基金】：宁夏自然科学基金资助项目(NZ15011)
【分类号】：S831

【相似文献】