2016年我国3株欧洲型猪繁殖与呼吸综合征病毒的分离鉴定及其全基因组特征分析

发布时间：2018-04-11 01:03

本文选题：猪繁殖与呼吸综合征病毒 + 基因型　；参考：《中国预防兽医学报》2017年07期

【摘要】：2016年,本实验室共检测到3株基因1型(欧洲型)猪繁殖与呼吸综合征病毒(PRRSV),分别命名为ZD1株、WK14株和WG9株,本研究对3株PRRSV进行了全基因组序列分析。遗传演化分析显示,3株PRRSV均属于基因1型I亚型,与基因1型代表株LV的核苷酸同源性为88.7%~88.9%,与基因2型代表株VR2332的核苷酸同源性为60.7%~60.8%。ORF3与ORF4推导氨基酸序列比对结果显示,ZD1株的ORF3提前26个氨基酸(aa)终止,这是首次报道基因1型PRRSV编码的结构蛋白存在提前终止现象,WK14株与WG9株在ORF3的245位存在1个氨基酸的缺失;3株PRRSV在ORF4的66位皆存在1个氨基酸的缺失。Simplot生物学重组软件分析表明,WK14株为重组病毒,供体病毒株为BJEU06-1,提供重组片段的病毒株为NVDC-FJ,重组位置位于第931~3 379位碱基处。ZD1株和WG9株未发现重组现象。本研究发现我国欧洲型PRRSV基因组在缺失、重组等方面正发生一些新的变化,有必要对该类病毒进行持续监测和深入研究。
[Abstract]:In 2016, three strains of porcine reproductive and respiratory syndrome virus type 1 (European type), named ZD1 strain WK14 and WG9 strain, were detected in our laboratory. Three PRRSV strains were sequenced.Genetic evolution analysis showed that all three PRRSV strains belonged to subtype I of gene 1.The nucleotide homology with LV, the representative strain of gene 1, was 88.788. 9, and the nucleotide homology with VR2332 of genotype 2 was the deduced amino acid sequence of 60.7%~60.8%.ORF3 and ORF4. The results showed that the ORF3 of ZD1 strain terminated ahead of 26 amino acids (aaa).This is the first time to report the early termination of structural proteins encoded by type 1 PRRSV and the presence of 1 amino acid deletion in ORF3 of WK14 strain and WG9 strain. 3 strains of PRRSV have 1 amino acid deletion in ORF4. The biological weight of Simplot is 1 amino acid deletion in ORF4.Group software analysis showed that WK14 strain was a recombinant virus.The donor strain was BJEU06-1, and the recombinant fragment was NVDC-FJ. The recombinant site was located at the 931t3379bp. ZD1 strain and WG9 strain had no recombination phenomenon.In this study, we found that some new changes are taking place in the deletion and recombination of the European PRRSV genome in China. It is necessary to continuously monitor and further study this kind of virus.
【作者单位】：中国农业科学院哈尔滨兽医研究所;山东信得生物科技有限公司;南阳师范学院;中国农业科学院上海兽医研究所;
【基金】：国家自然科学基金(31270045、31502097)
【分类号】：S852.651

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