抗菌肽JH-3治疗沙门氏菌感染的效果评价

发布时间：2018-04-11 19:08

本文选题：抗菌肽JH- + 沙门氏菌　；参考：《微生物学通报》2017年12期

【摘要】：【目的】沙门氏菌(Salmonella)是重要的人畜共患传染病原菌,其感染是引起世界性胃肠疾病的主要因素,全球每年大概有2 100万伤寒病例,给世界公共卫生带来严重威胁。目前抗生素滥用问题严峻,急需寻找一种抗生素的替代品。抗菌肽JH-3是本实验室分离并人工改造后具有广谱杀菌活性的小肽,以沙门氏菌标准菌株CVCC541为研究对象,在小鼠模型上评价抗菌肽JH-3治疗沙门氏菌感染的效果。【方法】在CVCC541感染BALB/c小鼠前3 d连续腹腔注射抗菌肽JH-3或环丙沙星(B3d,共计40 mg/kg)和感染后3 d连续注射JH-3或环丙沙星(P3d,共计40 mg/kg)进行治疗。【结果】发现环丙沙星预防组效果最佳,抗菌肽JH-3的预防组(B3d)效果较好,可显著保护小鼠免受致死剂量CVCC541的攻击,小鼠存活率高达100%,临床症状评分、血液和脏器荷菌数降低,小肠段病理变化减轻,效果与环丙沙星治疗组相当;而感染3 d后JH-3治疗效果较差,临床症状评分、脏器荷菌量以及小肠病理变化均显著高于3 d前预防组,但3 d后治疗组小鼠存活率为70%,仍明显高于单独感染组。【结论】系统评价了抗菌肽JH-3不同给药时间对沙门氏菌感染的治疗效果,预防性给药方式的抗菌作用最佳,与环丙沙星治疗效果相当,为新型抗菌药物的研究提供参考。
[Abstract]:[objective] Salmonella is an important zoonotic pathogen, and its infection is the main cause of gastrointestinal diseases worldwide. There are about 2 1 million cases of typhoid fever in the world every year, which pose a serious threat to public health in the world.At present, the problem of antibiotic abuse is serious, and it is urgent to find a substitute for antibiotics.Antimicrobial peptide JH-3 is a small peptide with broad-spectrum bactericidal activity after isolation and artificial modification in our laboratory. CVCC541, a standard strain of Salmonella, was used as the research object.To evaluate the efficacy of antimicrobial peptide JH-3 in the treatment of Salmonella infection in mice model. [methods] JH-3 or ciprofloxacin were injected intraperitoneally 3 days before CVCC541 infection in BALB/c mice for a total of 40 mg / kg) and JH-3 was injected continuously 3 days after infection.Ciprofloxacin P3d, 40 mg / kg, was treated. [results] it was found that ciprofloxacin prophylaxis group had the best effect.The prophylactic effect of antimicrobial peptide JH-3 was better than that of control group (B3d), which could significantly protect mice from lethal dose of CVCC541. The survival rate of mice was as high as 100. The clinical symptom score, the number of blood and organs were decreased, and the pathological changes of intestinal segment were alleviated.The effect was similar to that of ciprofloxacin group, but the effect of JH-3 was worse after 3 days of infection, and the clinical symptom score, organ load and intestinal pathological changes were significantly higher than those in the prevention group before 3 days.But after 3 days, the survival rate of mice in the treatment group was 70, which was still significantly higher than that in the single infection group. [conclusion] the therapeutic effect of antimicrobial peptide JH-3 on Salmonella infection was evaluated systematically, and the antibacterial effect of prophylactic administration was the best.The efficacy of ciprofloxacin is comparable to that of ciprofloxacin, which provides a reference for the study of new antimicrobial agents.
【作者单位】：河南科技学院动物科技学院;
【基金】：国家重点研发计划项目(No.2016YFD0500708-4) 国家自然科学基金面上项目(No.31672559) 第60批国家博士后科学基金项目(No.2016M600578)~~
【分类号】：S855.12

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