复合酶对几种饲料原料养分消化率的影响及其在猪饲料中的应用

发布时间：2018-04-12 17:47

本文选题：复合酶 + 体外消化　；参考：《沈阳农业大学》2016年硕士论文

【摘要】：本文采用体外消化试验和动物饲养试验,测定复合酶对不同饲料原料养分消化率和生长猪生产性能的影响。试验结果如下：1.体外消化试验。为探明复合酶对玉米、豆粕、麦麸、DDGS、棉粕、菜粕中养分消化率的影响,通过体外模拟胃部消化、小肠消化和后肠消化三步消化的试验方法测定上述饲料原料中干物质、能量、粗蛋白、粗纤维、中性洗涤纤维、酸性洗涤纤维、钙和磷的消化率,试验设加酶和不加酶两个处理,加酶组在待消化的饲料原料中加0.1%的同种商品型复合酶。试验结果表明,复合酶显著提高玉米中CP、P、Lys的消化率(P0.05),对干物质等其他指标消化率的影响差异不显著(P0.05),但均有提高趋势；复合酶极显著提高豆粕中P的消化率(P0.01),显著提高GE、DM、CP、CF、NDF、ADF和Cys的消化率(P0.05),对部分氨基酸消化率的影响差异不显著(P0.05),但均有提高趋势；复合酶极显著提高麦麸中DM和Met的消化率(P0.01),显著提高GE、P、CF、NDF、ADF、Thr(?)口Try的消化率(P0.05),对CP、Lys和Cys消化率的影响差异不显著(P0.05),但均有提高趋势；复合酶极显著提高DDGS中P和ADF消化率(P0.01),显著提高GE、CF和NDF的消化率(P0.05),对干物质等其他指标消化率的影响差异不显著(P0.05),但均有提高趋势；复合酶极显著提高棉粕中NDF和ADF消化率(P0.01),显著提高GE、CF、DM、CP、P、Met和Thr的消化率(P0.05),对Lys、Cys(?)Try消化率的影响差异不显著(P0.05),但均有提高趋势；复合酶极显著提高菜粕中P、NDF和ADF消化率(P0.01),显著提高GE、DM、CP、CF(?)Lys的消化率(P0.05),对部分氨基酸消化率的影响差异不显著(P0.05),但均有提高趋势；复合酶对不同饲粮同种营养物质消化率亦有较大差异。2.动物饲养试验。将体重25Kg左右的杜×长×大生长猪40头,按体重相近、公母各半原则分为2个处理,每处理5个重复,每个重复4头猪。对照组喂基础日粮,试验组在基础日粮中添加0.1%的复合酶。饲养试验持续时间为60天,试验开始和结束时空腹个体称重,试验期间按重复组统计耗料量计算猪的平均日增重(ADG)、料重比(F/G)、平均日采食量(ADFI)、腹泻率和血液生化指标。试验结果表明,日粮中加酶组与对照组相比,日增重显著提高3.57%(P0.05),采食量和料肉比降低均不显著(P0.05),腹泻率显著降低52.3%(P0.05)；添加复合酶后血清总蛋白、白蛋白、球蛋白均比对照组显著提高(P0.05),分别为8.25%、8.74%、7.69%；谷草转氨酶、碱性磷酸酶较对照组显著降低(P0.05),分别为4.52%、11.85%,谷丙转氨酶、乳酸脱氢酶降低不显著(P0.05)；血糖、血清胆固醇的含量均提高,但都不显著(P0.05),血清尿素氮较对照组显著降低8.02%(P0.05),血清甘油三酯降低不显著(P0.05)。综上所述,本研究选用的专用商品型复合酶能显著提高几种饲料原料中多数营养物质的消化率,能改善生长猪的生长性能和部分血液生化指标。
[Abstract]:In this paper, in vitro digestion test and animal feeding test were used to determine the effects of compound enzyme on nutrient digestibility of different feed materials and production performance of growing pigs.The test results are as follows: 1.In vitro digestion test.In order to find out the effect of compound enzyme on nutrient digestibility of corn, soybean meal, wheat bran DDGS, cottonseed meal and rapeseed meal, the dry matter and energy of the feedstuff were determined by three steps digestion of simulated stomach digestion, small intestine digestion and hindgut digestion in vitro.The digestibility of crude protein, crude fiber, neutral detergent fiber, acid detergent fiber, calcium and phosphorus were studied.The results showed that the digestibility of CPP Lys in maize was significantly increased by the combined enzyme, but there was no significant difference on the digestibility of other indexes, such as dry matter, but there was a tendency to increase the digestibility.The digestibility of P in soybean meal and the digestibility of Cys and Cys were significantly increased by the compound enzyme. The difference on the digestibility of some amino acids was not significant, but there was a tendency to increase.The digestibility of DM and Met in wheat bran was significantly increased by the combined enzyme, and the digestibility of DM and Met in wheat bran was significantly increased.The digestibility of oral Try was not significantly different from that of Cys, but there was a tendency to increase.The digestibility of P and ADF in DDGS and the digestibility of NDF and CF were significantly increased by the compound enzyme, but there was no significant difference on the digestibility of other indexes, such as dry matter, but there was a tendency to increase.The digestibility of PnDF and ADF in rapeseed meal and the digestibility of P0.05N in Ge-DMN were significantly increased by the compound enzyme, but there was no significant difference on the digestibility of some amino acids, but there was a tendency to increase the digestibility of PnDF and ADF in rapeseed meal.The digestibility of the same nutrients in different diets was also significantly different from that of the enzyme. 2. 2.Animal feeding experiment.According to the principle of weight similarity, male and female were divided into 2 treatments, 5 replicates per treatment, 4 pigs per replica.The control group was fed with basal diet, and the experimental group was supplemented with 0.1% compound enzyme in the basal diet.The feeding experiment lasted for 60 days, and the fasting individuals weighed at the beginning and the end of the experiment. During the experiment, the average daily gain (ADG), feed weight ratio (FR), ADFI (ADFI), diarrhea rate and blood biochemical index of pigs were calculated according to the feed consumption of repeated groups.The results showed that compared with the control group, the daily gain of the enzyme added group increased 3.57% significantly, the feed intake and the feed / meat ratio were not significantly decreased, the diarrhea rate was significantly decreased by 52.3% P0.05, and the serum total protein and albumin were significantly decreased after adding the compound enzyme.The serum urea nitrogen was significantly lower than that of the control group (P 0.05), and the serum triglyceride was not significantly decreased (P 0.05).To sum up, the special commercial compound enzyme can significantly increase the digestibility of most nutrients in several feed materials, improve the growth performance and some blood biochemical indexes of growing pigs.
【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S828.5

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