近年来湖南省伪狂犬病血清学调查及一例伪狂犬病的诊断

发布时间：2018-04-12 17:41

本文选题：伪狂犬病 + 湖南省　；参考：《湖南农业大学》2015年硕士论文

【摘要】：伪狂犬病是由伪狂犬病毒引起的高接触性传染病,对养猪业的危害极大,为了解伪狂犬病在湖南省的防控与流行情况,从而有效的预防和控制伪狂犬病的发生,2013年1月～2015年9月来自于湖南省长沙、株洲、湘潭、衡阳、益阳、岳阳、邵阳、郴州、娄底和常德10个地区111个猪场的3304份血清采用IDEXX公司的gE-ELISA方法检测伪狂犬病gE抗体,结果显示湖南省猪场阳性率达到54.05%,血清样品阳性率达到23.94%,其中衡阳、长沙、郴州、湘潭、益阳和邵阳地区的猪场伪狂犬病野毒感染阳性率较高,分别为90.91%、66.67%、66.67%、62.50%、55.56%和50.00%；不同年份血清伪狂犬病gE抗体检测结果显示从2013年到2015年血清样品伪狂犬病gE抗体阳性率从9.04%逐年升高到36.49%,多个地区在2014年和2015年呈现出高的伪狂犬病野毒感染阳性率。在对湖南省2013年到2015年伪狂犬病血清流行病学进行调查后可以发现近年湖南省伪狂犬病的流行较为严重,并且近两年有抬头的趋势。为对某规模化猪场疑似爆发的伪狂犬病进行诊断,采取措施控制疫情,减少猪场损失,对该猪场疑似伪狂犬病病例进行采样、PCR鉴定、伪狂犬病毒分离和毒力鉴定后,该猪场被确诊为伪狂犬病毒强毒株感染,给予猪场防控建议并迅速控制疫情。通过分析该强毒株的gE基因序列,结果发现该毒株与国内的BJ-YT株、TJ株、Ea株的同源性较高,核苷酸同源性在99.7%以上,氨基酸同源性在99.6%以上,系统进化树上也位于同一大的分支,说明该猪场流行的伪狂犬病毒与国内别的地区流行的毒株亲缘关系较近,这为合理选择伪狂犬病毒疫苗对猪群免疫提供了理论基础。
[Abstract]:Pseudorabies is a highly contagious disease caused by pseudorabies virus, which is harmful to pig industry. In order to understand the prevention, control and epidemic situation of pseudorabies in Hunan Province,From January 2013 to September 2015, from Changsha, Zhuzhou, Xiangtan, Hengyang, Yiyang, Yueyang, Shaoyang, Chenzhou,3304 sera collected from 111 pig farms in Loudi and Changde were tested for pseudorabies GE antibody by gE-ELISA method of IDEXX Company. The results showed that the positive rate of pig farms in Hunan Province reached 54.05 and the positive rate of serum samples reached 23.94. Among them, Hengyang, Changsha, Chenzhou, Xiangtan,The positive rate of pseudorabies virus infection in pig farms in Yiyang and Shaoyang areas was high.The positive rate of canine wild-virus infection.After investigating the seroepidemiology of pseudorabies in Hunan province from 2013 to 2015, we can find that the prevalence of pseudorabies in Hunan Province is more serious in recent years, and the trend of increasing in recent two years.In order to diagnose the suspected outbreak of pseudorabies in a large scale pig farm, measures were taken to control the epidemic situation and reduce the loss of the pig farm. The suspected pseudorabies cases in the pig farm were identified by PCR, the pseudorabies virus was isolated and the virulence of the pseudorabies virus was identified.The pig farm was diagnosed as pseudorabies virus virulent infection.By analyzing the GE gene sequence of the virulent strain, it was found that the strain had high homology with BJ-YT strain TJ strain EA, nucleotide homology was more than 99.7%, amino acid homology was more than 99.6%, and the phylogenetic tree was also located in the same big branch.The results showed that the relationship between pseudorabies virus and other strains in other regions of China was close, which provided a theoretical basis for the rational selection of pseudorabies virus vaccine against swine population immunization.
【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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