D群猪链球菌疫苗免疫条件优化

发布时间：2018-04-13 04:19

本文选题：猪链球菌 + 凝集效价　；参考：《大连工业大学》2015年硕士论文

【摘要】：猪链球菌为革兰氏阳性菌,是一种能够引起人和动物感染的动物源性细菌,可引起脑膜炎、心肌炎、败血症等症状。此种病分布比较广泛,随着养猪业工厂化的不断进步,这种病已升级到危害养猪业的一种严重病症。这种疾病四季均会发生,病发率和病死率相对其他疾病高,会带给养殖户巨大的经济损失。不仅如此,该病还能导致人感染,甚至引起人的死亡,严重地威胁着人类的公共卫生安全。目前对于猪链球菌病的防治仍主要依赖抗生素的应用,但长期大量的滥用抗菌药物,导致耐药菌株的不断出现,带来治疗上的困难。疫苗在一些疾病预防上发挥了难以取代的作用,可靠、安全的疫苗,能够使动物体内抗体水平升高来抵抗细菌的感染,以便能够达到预防目的;此外,也可通过疫苗的应用进行主动免疫治疗。近来有关D群猪链球菌疫苗的研究较少。本实验目的在于应用正交设计,优化D群猪链球菌疫苗免疫条件,旨在为猪链球菌的主动免疫预防提供实验依据。本实验通过选择抗原剂量,免疫途径和免疫时间间隔三个因素,每个因素设三个水平,按照L9(34)正交表免疫动物,测定在不同的组合免疫下,家兔血清中凝集效价、IgG含量,家兔的脾重系数、特异B细胞克隆计数以及B细胞增殖百分比,从而选出最佳的免疫组合。通过实验结果分析,考虑到实验结果在现实环境中的应用效果,使用成本,以及免疫便利性等问题,最终选择最佳的免疫条件组合是:抗原剂量选择为1×108cfu/mL,免疫途径选择皮下注射,免疫时间间隔为两周。
[Abstract]:Streptococcus suis is a Gram-positive bacteria, which can cause human and animal infection, can cause meningitis, myocarditis, septicemia and other symptoms.The disease is widely distributed, and with the continuous progress of the factory industry, the disease has been upgraded to a serious disease that endangers the pig industry.This disease will occur all four seasons, the incidence rate and mortality rate is higher than other diseases, will bring farmers a huge economic loss.Moreover, the disease can cause human infection and even death, which seriously threatens the public health safety.At present, the prevention and treatment of Streptococcus suis is still mainly dependent on the use of antibiotics, but a large number of long-term abuse of antimicrobial agents, leading to the emergence of drug-resistant strains, leading to difficulties in treatment.Vaccines play an irreplaceable role in the prevention of some diseases. A reliable, safe vaccine can increase the level of antibodies in animals to resist bacterial infections, so as to achieve the purpose of prevention.Active immunotherapy can also be carried out through the application of vaccines.Recently, there has been little research on group D streptococcus suis vaccine.The purpose of this experiment was to optimize the immunization conditions of group D streptococcus suis vaccine by orthogonal design, and to provide experimental basis for active immunization prevention of Streptococcus suis.In this experiment, we selected three factors of antigen dosage, immune pathway and immune time interval, each factor was divided into three levels, according to L9 / 34) orthogonal surface immunized animals, the agglutination titer IgG content in rabbit serum was determined under different combinations of immunization.The spleen weight coefficient, specific B cell clone count and the percentage of B cell proliferation in rabbits were selected to select the best immune combination.Through the analysis of the experimental results, considering the application effect of the experimental results in the real environment, the cost of use, and the convenience of immunization, and so on,The optimal immune conditions were as follows: the dose of antigen was 1 脳 108cfu-mL, the immune pathway was subcutaneously injected, and the time interval of immunization was two weeks.
【学位授予单位】：大连工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.4

【相似文献】