脂联素对酮病奶牛中性粒细胞自噬相关基因mRNA表达的影响

发布时间：2018-04-13 18:48

本文选题：高BHBA + ADPN　；参考：《中国兽医学报》2017年01期

【摘要】：通过模拟向高BHBA的中性粒细胞培养液中添加不同质量浓度的脂联素(ADPN),运用荧光定量PCR技术,分别检测中性粒细胞自噬相关基因mRNA的表达水平。结果显示:添加高BHBA(4.8mol/L)的中性粒细胞培养6h后,与对照组相比,自噬相关基因LC3B、Beclin、ULK1的mRNA表达水平显著升高(P0.01),SQSTM1(p62)、mTOR的mRNA表达水平显著下降(P0.01);添加不同质量浓度的ADPN(25,50,100μg/L)后,相对于高BHBA组,中性粒细胞自噬相关基因LC3B、Beclin、ULK1的mRNA表达水平显著下调,且在ADPN 50μg/L时,具有极显著差异性(P0.01);此外SQSTM1、mTOR的mRNA表达水平显著升高。结果表明:BHBA可增加中性粒细胞自噬相关基因的表达,ADPN可抑制高BHBA引起的中性粒细胞细胞程序性死亡,进一步说明ADPN对调控酮病奶牛中性粒细胞免疫抑制具有重要的价值意义。
[Abstract]:Through the simulation of neutrophils to high BHBA culture medium added with different concentrations of adiponectin (ADPN), using fluorescence quantitative PCR technology, the expression level of mRNA gene is associated with neutrophil autophagy were detected. The results showed that adding high BHBA (4.8mol/L) of the neutrophil 6h after culture, compared with the control group. Autophagy related genes LC3B, Beclin, the expression level of ULK1 mRNA increased significantly (P0.01), SQSTM1 (p62), the expression level of mTOR mRNA decreased significantly (P0.01); adding different concentrations of ADPN (25,50100 g/L), compared to the BHBA group, the neutrophil autophagy related gene LC3B, Beclin expression level. ULK1 mRNA and ADPN were significantly reduced, in 50 g/L, with significant difference (P0.01 SQSTM1); in addition, the expression level of mTOR mRNA increased significantly. The results show that BHBA can increase the expression of neutrophil cell autophagy related genes, ADPN can inhibit BHBA induced high The programmed cell death of neutrophils further indicates that ADPN is of great value in regulating the immune suppression of neutrophils in cows with ketosis.

【作者单位】：吉林大学动物医学学院;
【基金】：国家自然科学基金资助项目(31372494,C180802)
【分类号】：S858.23

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