湖南滨湖水牛卵泡动态发育及生殖激素变化的研究

发布时间：2018-04-14 00:15

  本文选题：滨湖水牛 + 卵泡　； 参考：《湖南农业大学》2015年硕士论文

【摘要】：为了提高滨湖水牛繁殖力,本研究运用同期发情技术和B超诊断分析了滨湖水牛卵泡发育动态变化和生殖激素含量变化。1 湖南滨湖水牛卵泡发育动态变化的研究本研究采用同期发情技术处理滨湖水牛,利用B超检测滨湖水牛卵泡动态发育情况。研究结果表明：(1)卵泡波出现时间：2波周期青年水牛的第1波和第2波分别在1.5d,8.2d出现；经产水牛则在1.0d,12.3d出现,两者差异不显著(P0.05)。3波周期中第1波、第2波、第3波分别出现在1.0d,7.5d和15.0d。(2)卵泡波持续时间：2波周期中,青年牛第1波、第2波分别持续7.16d、10.33d；经产牛第1波、第2波分别为9.33d和11.13d。3波周期中3个波各持续6.5、8.0和9.0d。2波或3波周期,各波持续的天数间均无显著性差异(P0.05)。(3)发情周期内征集的卵泡总数：经产牛为16.46个,要显著高于青年牛的12.48个(P0.05)。(4)周期内选择的卵泡总数：经产牛为7.96个,与青年牛的7.30个差异不显著(P0.05)。(5)优势卵泡最大直径：2波周期中青年牛第1、2波优势卵泡最大直径分别为8.22mm和9.81mm,经产牛第1、2波优势卵泡最大直径分别为7.85mm和7.54mm,两者差异不显著(P0.05)。3波周期中的滨湖青年母水牛的第1、2、3波优势卵泡最大直径分别为7.54mm、10.33mm和8.32mm。(6)各级卵泡数：青年水牛、经产水牛之间在左、右卵巢上各类型卵泡的数量统计均无明显差异(P0.05)。2滨湖水牛生殖激素变化的研究本研究运用放射免疫技术分析了发情周期内生殖激素变化。研究结果表明：(1)从发情前2d到出现发情,FSH持续升高,发情后1d出现最高峰(3.5mIU/mL),然后持续回落。按发情周期各阶段分析,发情中期FSH水平值最高,且显著高于发情前期、发情后期和间情期(P0.05)；(2)从发情前期到发情第3d,LH水平呈现一个缓慢上升的过程,其中第3d达到22.13mIU/mL,第6d降到16.12mIU/mL;然后先缓慢升高再下降,第10d达到最低值15.17mIU/mL;之后缓慢升高,分别在14d、16d出现两个小高峰,峰值分别为20.71和22.17mIU/mL,第18d出现最高值23.24mIU/mL。发情期三个阶段(前期、中期和后期)的LH水平分别为20.65、20.02和21.29mIU/mL,但差异不显著(P0.05),但都要显著高于间情期的17.24mIU/mL(Pp0.05)。(3)发情前2d,E2水平最低(20.43 pg/mL),然后持续缓慢升高,发情第6d达到一个小高峰(72.88 pg/mL);之后缓慢下降,第15d到达低值(39.90 pg/mL);之后迅速上升,第19d达到最大峰值(118.32 pg/mL),随后又迅速下降到40pg/mL以下。发情前期E2水平(67.83 pg/mL)显著高于发清中期(51.01 pg/mL)、发情后期(56.33pg/mL)和间情期(53.74 pg/mL) (P0.05)。而其他各阶段的E2水平差异不显著(Pp0.05)。(4)发情前2d,P_4水平急剧下降,发情当天(0d)降到最低值(0.37pg/mL),然后开始上升(出现三个峰值,即第7d为6.79pg/mL,10d达到最大值8.96pg/mL,14d为7.77pg/mL);随后迅速下降。发情中期(1.87pg/mL)和发情后期(2.62pg/mL) P_4水平最低,两者差异不显著和(P0.05),但均低于发情前期(4.13pg/mL和间情期(5.71pg/mL),且差异显著(P0.05),间情期P_4水平高于发情前期,且差异显著(P0.05)。
[Abstract]:In order to improve Binhu buffalo Binhu buffalo fecundity, analysis on follicular dynamics and changes of reproductive hormones in Hunan Binhu buffalo.1 dynamic changes of follicular development by this study of estrus synchronization treatment Binhu water cow estrus synchronization and ultrasound diagnosis by this study, the use of ultrasound in Binhu buffalo follicular dynamics development. The results show that: (1) follicular wave time: first wave and second wave 2 wave period youth buffalo respectively in 1.5D, 8.2d; Buffalo multiparous appeared in 1.0d, 12.3d, had no significant difference (P0.05).3 wave period in the first wave, the second wave, the third wave were found in 1.0d, 7.5d and 15.0d. (2) follicular wave duration: 2 wave cycle, the first wave of the second wave of young cattle, respectively for 7.16d, 10.33d; multiparous cattle first waves, second waves are respectively 9.33d and 11.13d.3 in the 3 wave of the wave period of persistent 6.5,8.0 and 9.0d.2 wave Or 3 wave period, there were no significant differences in the number of days between continuous wave (P0.05). (3) the total number of follicles in the estrous period for 16.46 multiparous: cattle, was significantly higher than that of the 12.48 young cattle (P0.05). (4) the total number of follicular period selection: 7.96 multiparous cows 7.30, with no significant difference between the young cattle (P0.05). (5) the maximum diameter of the dominant follicle: 2 wave period in young cattle 1,2 wave maximum dominant follicle diameter were 8.22mm and 9.81mm, the 1,2 wave of the dominant follicle multiparous cows maximum diameter were 7.85mm and 7.54MM, the difference was not significant (P0.05) in Binhu the young mother of buffalo.3 in the period of wave 1,2,3 wave maximum diameter of dominant follicles were 7.54MM, 10.33mm and 8.32mm. (6): the number of follicles of young buffalo, buffalo in between the left and right ovarian follicle number statistics of various types were not significantly different (P0.05).2 change of reproductive hormone in Binhu Buffalo This study used radioimmunoassay analyses the change of reproductive hormones during the estrous cycle. The results show that: (1) from 2D to appear before estrus estrus, FSH continues to rise, the peak estrus after 1D (3.5mIU/mL), and then continued to fall. According to the analysis of each stage of the estrous cycle, the highest level of estrus at metaphase FSH, and was significantly higher than that of proestrus, estrus and diestrus stage (P0.05); (2) from proestrus estrus to 3D, LH levels showed a slow rise in the process, the 3D reached 22.13mIU/mL, 6D to 16.12mIU/mL; and then slowly increased and then decreased, the 10d reached the lowest value after 15.17mIU/mL; increased slowly, respectively in 14d, 16d two small peaks, the peak is 20.71 and 22.17mIU/mL respectively, the 18D peak 23.24mIU/mL. estrus three stages (early, middle and late) LH levels were respectively 20.65,20.02 and 21.29mIU/mL, but the difference 涓嶆樉钁，

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