不同日龄仔猪外周血T淋巴细胞的检测与分析

发布时间：2018-04-14 15:34

本文选题：仔猪 + T淋巴细胞亚群　；参考：《浙江农业学报》2017年08期

【摘要】：为建立健康仔猪外周血淋巴细胞亚群的临床参考范围,试验分析不同生长阶段仔猪的免疫系统功能水平,分别在健康仔猪断奶后第1、7、21和35天采取外周血,分离外周血单个核细胞,采用三色流式细胞术的方法检测各类主要T淋巴细胞亚群所占外周血单个核细胞的比例,及其占CD3~+T淋巴细胞亚群中的比例,计算CD4~+/CD8~+的比值。结果表明,随着日龄的增加公母仔猪CD3~+T淋巴细胞亚群占外周血单个核细胞的比例逐渐升高,分别从(38.00±10.94)%和(38.17±15.39)%上升至(55.43±12.30)%和(51.57±10.60)%。在断奶后1 d和7 d公母仔猪CD3~+T淋巴细胞比例无显著性差异(P0.05),至断奶后21 d母仔猪CD3~+T淋巴细胞比例显著增加(P0.05),公仔猪至35 d时CD3~+T淋巴细胞比例显著增加(P0.05)。随着日龄的增长,公母仔猪CD4~+T淋巴细胞亚群占外周血单个核细胞的比例分别由(10.29±2.71)%和(10.3±2.17)%上升至(22.2±5.87)%和(23.4±6.84)%。公母仔猪CD4~+T淋巴细胞的比例逐渐升高,且各个时间点公母仔猪结果无明显差异(P0.05)。公母仔猪CD8~+T淋巴细胞占外周血单个核细胞的比例在各时间点分别为(23.80±6.67)%、(17.14±10.64)%、(25.46±12.32)%、(33.33±8.93)%和(28.55±6.75)%、(23.62±11.51)%、(37.37±13.18)%、(38.31±15.66)%。随着日龄的增加公母断奶仔猪CD8~+T淋巴细胞呈现先下降后升高的趋势。断奶仔猪各淋巴细胞亚群分布会随着断奶时间的增加而呈现不同的变化,并且存在着性别上的差异。结合两种不同的细胞圈定方法,能够更准确地反映出仔猪体内各淋巴细胞亚群数量和比例的变化。
[Abstract]:In order to establish the clinical reference range of peripheral blood lymphocyte subsets of healthy piglets, the immune system function of piglets at different growth stages were analyzed, and peripheral blood was taken on the 1st and 35th day after weaning, respectively.Peripheral blood mononuclear cells (PBMC) were isolated. The percentage of major T lymphocyte subsets in peripheral blood mononuclear cells (PBMC) and the percentage of CD3- T lymphocyte subsets (CD3- T lymphocyte subsets) in peripheral blood mononuclear cells (PBMC) were detected by three color flow cytometry. The ratio of CD4 to / / CD8 ~ was calculated.The results showed that the percentage of CD3- T lymphocyte subsets in peripheral blood mononuclear cells (PBMC) of male and female piglets gradually increased with the increase of age, from 38.00 卤10.94% and 38.17 卤15.39% to 55.43 卤12.30% and 51.57 卤10.60%, respectively.There was no significant difference in the proportion of CD3 ~ T lymphocytes between male and female piglets on day 1 and 7 after weaning. The percentage of CD3 ~ T lymphocytes in female piglets increased significantly at 21 days after weaning, and the proportion of CD3 ~ T lymphocytes in female piglets increased significantly at 35 days after weaning, and the proportion of CD3 ~ T lymphocytes in female piglets increased significantly at 35 days after weaning.The percentage of CD4T lymphocyte subsets in peripheral blood mononuclear cells (PBMC) of male and female piglets increased from 10.29 卤2.71% and 10.3 卤2.17% to 22.2 卤5.87% and 23.4 卤6.84%, respectively.The percentage of CD4 ~ T lymphocytes in male and female piglets increased gradually, and there was no significant difference in the results of male and female piglets at different time points.The percentage of CD8T lymphocytes in peripheral blood mononuclear cells (PBMC) of male and female piglets was 23.80 卤6.67g / L at each time point, respectively. The ratio of CD8T lymphocytes to peripheral blood mononuclear cells (PBMC) was 17.14 卤10.64 and 25.46 卤12.32 卤33.33 卤8.93% and 28.55 卤6.75 + 23.62 卤11.511,37.37 卤13.1838.31 卤15.66m respectively.With the increase of age, CD8 ~ T lymphocytes of male and female weaned piglets decreased first and then increased.The distribution of lymphocyte subsets in weaned piglets varied with the increase of weaning time, and there were gender differences.Combined with two different methods of cell delineation, the number and proportion of lymphocyte subsets in piglets can be more accurately reflected.
【作者单位】：甘肃农业大学动物医学院;中国农业科学院兰州畜牧与兽药研究所甘肃省中兽药工程技术研究中心;
【基金】：公益性行业专项(201303040-15) 国家自然科学基金面上项目(31472233) 甘肃省科技支撑计划(1604NKCA069-03)
【分类号】：S828

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