VEGF在绵羊性成熟前后睾丸组织中表达水平的比较分析

发布时间：2018-04-14 19:06

本文选题：血管内皮生长因子 + 性成熟前后　；参考：《中国兽医科学》2017年02期

【摘要】：为探索血管内皮生长因子(VEGF)在绵羊睾丸发育和精子发生过程中的作用,本研究以绵羊性成熟前后睾丸组织为研究对象,采用RT-PCR和Western-blot方法检测VEGF m RNA及其蛋白在绵羊性成熟前后睾丸组织中的表达差异,利用SSPS 18.0对数据进行比较分析。结果显示,VEGF m RNA及其蛋白在绵羊性成熟前后睾丸组织中均有表达,二者在性成熟后睾丸中的表达量明显高于性成熟前睾丸(P0.05),且VEGF m RNA与蛋白的表达趋势趋于一致。由此可见,VEGF在绵羊睾丸发育和精子发生过程中具有促进作用,这为进一步探索VEGF在睾丸中的作用机制奠定了一定的理论基础。
[Abstract]:In order to explore the role of vascular endothelial growth factor (VEGF) in the development of testis and spermatogenesis in sheep, the testis of sheep before and after sexual maturation were studied.RT-PCR and Western-blot methods were used to detect the expression of VEGF m RNA and its protein in testis of sheep before and after sexual maturation. SSPS 18.0 was used to compare the data.The results showed that VEGFM RNA and its protein were expressed in the testis of sheep before and after sexual maturation. The expression of VEGFM and its protein in the testis after sexual maturation was significantly higher than that in the testis before and after sexual maturation, and the expression trend of VEGF m RNA and protein tended to be the same.It can be seen that VEGF plays an important role in the development of testis and spermatogenesis in sheep, which lays a theoretical foundation for further exploring the mechanism of VEGF in testis.
【作者单位】：吉林农业大学动物科学技术学院;
【基金】：吉林省科技发展计划项目(20160204018NY) 吉林省现代肉羊产业技术体系项目(201641)
【分类号】：S826

【相似文献】