两株生物学特性不同的新城疫病毒La Sota毒株诱导DF-1细胞天然免疫反应的比较

发布时间：2018-04-15 21:36

本文选题：新城疫病毒 + 天然免疫　；参考：《畜牧与兽医》2017年06期

【摘要】：为探讨两株不同生物学特性的新城疫病毒La Sota毒株(La Sota参考毒株AV1615;La Sota突变株P2M1)引起的宿主天然免疫反应的差异,将两个毒株分别感染鸡胚成纤维细胞(DF-1),Real-time PCR及Western blot检测TLR3、TLR7、MDA5等受体的mRNA及蛋白表达;ELISA及免疫荧光检测细胞核转录因子IRF3的含量及其磷酸化水平;蛋白芯片检测40种细胞因子的分泌水平,并用ELISA验证部分与炎症、抗病毒等相关的细胞因子在细胞上清中的含量差异。结果显示,La Sota参考毒株AV1615感染细胞后,诱导产生的TLR3、TLR7、MDA5的mRNA及蛋白含量显著高于La Sota突变株P2M1(P0.05)。La Sota感染细胞后12 h,IRF3及其磷酸化水平、细胞上清中大部分细胞因子(31/40)的表达高于P2M1感染组。结果说明,两株La Sota毒株在诱导DF-1细胞的天然免疫反应方面存在差异,这可能是La Sota P2M1在鸡胚中更容易增殖的原因。
[Abstract]:To investigate the difference of host innate immune response induced by two different biological characteristics of Newcastle disease virus La Sota strain La Sota reference strain AV1615 La Sota mutant P2M1.Real-time PCR and Western blot were used to detect the expression of mRNA and protein of TLR3 and TLR7MDA5, respectively. The level of nuclear transcription factor IRF3 and its phosphorylation were detected by immunofluorescence.Protein chip was used to detect the secretion level of 40 cytokines, and ELISA was used to verify the difference of cytokines in the supernatant of some cytokines, such as inflammation, antivirus and so on.The results showed that the mRNA and protein contents of TLR3 + TLR7MDA5 induced by AV1615, a reference strain of La Sota, were significantly higher than those of P2M1(P0.05).La Sota, a mutant strain of La Sota, at 12 h after infection, and the level of IRF3 and phosphorylation of TLR3 was significantly higher than that of P2M1(P0.05).La Sota.The expression of most cytokines (31 / 40) in the supernatant was higher than that in the P2M1 infected group.The results showed that there was a difference between the two strains in inducing the innate immune response of DF-1 cells, which may be the reason why La Sota P2M1 proliferates more easily in chicken embryos.
【作者单位】：南京农业大学动物医学院;江苏省农业科学院动物免疫工程研究所;
【分类号】：S852.65

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