当归补血汤提取物亚慢性毒性试验研究

发布时间：2018-04-16 12:14

本文选题：当归补血汤 + 大鼠　；参考：《中国畜牧兽医》2017年10期

【摘要】：试验旨在评价当归补血汤提取物安全性,为临床安全用药提供试验依据。选取120只SD大鼠(雌雄各半)随机分为高、中、低剂量组和空白对照组,药物组分别按60、30、15 g/kg体重剂量灌胃给药,空白对照组给予等体积蒸馏水,连续给药28 d,停药后观察14 d。结果显示,给药结束时,高剂量组雄性大鼠出现体重增长减缓(P0.01)和饲料消耗量降低(P0.01),雄性大鼠白细胞数(WBC)、淋巴细胞(Lym)均显著升高(P0.05),丙氨酸氨基转移酶(ALT)、总胆固醇(TC)、葡萄糖(GLU)均极显著降低(P0.01),血小板(PLT)显著减少(P0.05);雌性大鼠红细胞(RBC)、血红蛋白(HGB)、ALT均极显著减少(P0.01)。中剂量组大鼠ALT和碱性磷酸酶(ALP)显著或极显著降低(P0.05;P0.01)。停药第14天雄性大鼠网织红细胞(RET)显著增多(P0.05),雌性大鼠HGB显著减少(P0.05)。停药后可恢复正常,其余相关检测指标均未见异常。各脏器组织病理学检查均未见异常。结果表明当归补血汤提取物毒性低,可以安全用于动物。
[Abstract]:The purpose of the experiment was to evaluate the safety of Danggui Buxue decoction extract and to provide experimental basis for clinical safety drug use.120 Sprague-Dawley rats (half male and female) were randomly divided into three groups: high, middle, low dose and blank control group. The drug group was given intragastrically at a body weight dose of 60 ~ 30g / 15 g/kg. The blank control group was given distilled water of the same volume for 28 days and observed for 14 days after withdrawal.The results showed that, at the end of the administration,In the high dose group, the weight gain of male rats decreased P0.01) and feed consumption decreased P0.01a. The white blood cell count of male rats increased significantly (P0.05), and the alanine aminotransferase (alt), total cholesterol (TCU) and glucose (GLU) decreased significantly.P0. 01, PLT) significantly decreased P0. 05%, while in female rats, RBC, HGBM and alt were significantly decreased in P0. 01%, and in female rats, P0. 01%, P 0. 01, P 0. 01, P 0. 01, P 0. 01, P 0. 01, respectively.In the middle dose group, ALT and alkaline phosphatase (ALP) decreased significantly or extremely significantly.On the 14th day after withdrawal, the reticulocyte (RET) of male rats increased significantly, while the HGB of female rats decreased significantly.After withdrawal, the drug can return to normal, and the other related indicators are not abnormal.No abnormality was found in histopathological examination of all organs.The results showed that the extract of Danggui Buxue decoction had low toxicity and could be safely used in animals.
【作者单位】：四川省畜牧科学研究院兽药研究所;动物遗传育种四川省重点实验室;
【基金】：四川省科技支撑计划(2015NZ0018) 四川省基本科研业务费项目(SASA2013B07) 四川省财政运行专项(SASA2014CZYX010)
【分类号】：S853.7

【相似文献】