植物乳杆菌-泰山松花粉多糖微胶囊对小鼠生长和肠道环境的影响

发布时间：2018-04-17 02:05

  本文选题：植物乳杆菌 + 泰山松花粉多糖　； 参考：《动物营养学报》2017年10期

【摘要】：植物乳杆菌作为微生态饲料添加剂饲喂后易受消化道胃酸、胆汁酸和消化酶等不利因素的影响。泰山松花粉多糖(TPPPS)作为一种水溶性植物多糖也易受到胃酸和酶的破坏。为了保护植物乳杆菌和TPPPS的生物活性,并探索二者之间是否存在协同作用。本研究以植物乳杆菌和TPPPS为芯材制备微胶囊,通过正交试验对包埋工艺进行优化,并对最佳条件下制备的微胶囊进行性能检测。选取体重20 g左右无特定病原体(SPF)小鼠48只,随机分为4组,每组12只。4组小鼠每天分别口服饲喂1 m L的植物乳杆菌、植物乳杆菌+0.5%TPPPS、微胶囊、磷酸盐缓冲液(PBS)。饲养试验42 d。每隔1周各组随机取3只小鼠称重记录,剖检后无菌采集肠道组织,测定小肠绒毛和隐窝发育差异及乳酸菌和大肠杆菌定植情况。结果显示:1)微胶囊包埋率达81.6%,粒径为84.3μm。在模拟胃液中处理120 min植物乳杆菌存活率为62.36%,37℃条件下存储120 d存活率达45.9%。2)与PBS组相比,植物乳杆菌、植物乳杆菌+0.5%TPPPS、微胶囊均能不同程度促进小鼠体重增加,提高小肠中乳酸菌数量,降低大肠杆菌数量,改善小肠绒毛和隐窝发育,微胶囊效果最优。结果提示,植物乳杆菌协同TPPPS使用的益生效果显著优于单独的植物乳杆菌,二者制备的微胶囊能够进一步提高其益生效果。
[Abstract]:Lactobacillus plantarum, as a microecological feed additive, is susceptible to adverse factors, such as gastric acid, bile acid and digestive enzymes.As a kind of water soluble plant polysaccharides, TPPPSs of Pinus tabulaeformis pollen are also easily damaged by gastric acid and enzyme.In order to protect the biological activity of Lactobacillus plantarum and TPPPS, and explore the synergy between them.In this study, microcapsules were prepared with Lactobacillus plantarum and TPPPS as the core material. The embedding process was optimized by orthogonal test, and the properties of the microcapsules prepared under the optimum conditions were tested.Forty-eight mice weighing about 20 g without specific pathogen were randomly divided into 4 groups. Each group of 12 mice were given 1 mL Lactobacillus plantarum, 0.5 TPPPS, microcapsule, and phosphate buffer solution PBSN.Feeding experiment 42 days.Every 1 week, 3 mice were randomly selected for weighing records. The intestinal tissues were collected without bacteria after dissection, and the differences in the development of villus and crypt and the colonization of lactic acid bacteria and Escherichia coli were measured.The results showed that the embedding rate of microcapsules was 81.6 渭 m and the particle size was 84.3 渭 m.The survival rate of Lactobacillus plantarum treated in simulated gastric juice for 120 min was 62.36 鈩，

本文编号：1761565