HGT KAPs基因家族在辽宁绒山羊皮肤毛囊中的表达研究

发布时间：2018-04-17 02:31

本文选题：辽宁绒山羊 + HGT　；参考：《中国畜牧杂志》2017年02期

【摘要】：为进一步探索高甘氨酸/酪氨酸角蛋白关联蛋白基因家族(HGT KAPs)与辽宁绒山羊羊绒品质——绒毛纤维直径的关系,本研究运用半定量RT-PCR检测KAP6-1.2、KAP6.2、KAP7.1、KAP8.1和KAP8.2在皮肤及各组织中的表达,通过原位杂交对其进行定位分析,采用实时荧光定量PCR检测兴盛期、退行期基因的相对表达量。结果表明:5个基因特异地表达于皮肤,在初、次级毛囊的皮质层、内根鞘中有明显的表达信号。KAP6-1.2和KAP8.1在兴盛期初次级毛囊的表达量差异不显著,KAP6.2在兴盛期、退行期初级毛囊的表达量均高于次级毛囊,而KAP7.1、KAP8.2在兴盛期次级毛囊的表达量高于初级毛囊,而退行期与之相反。因此推测,HGT KAPs基因家族在调控辽宁绒山羊绒毛纤维直径方面具有重要的作用,是编码羊绒结构蛋白的基因。
[Abstract]:In order to further explore the relationship between high glycine / tyrosine keratin associated protein gene family (HGT KAPs) and cashmere quality-chorionic fiber diameters in Liaoning cashmere goats, the expression of KAP6-1.2KAP6.2KAP7.1KAP8.1 and KAP8.2 in skin and tissues were detected by semi-quantitative RT-PCR.In situ hybridization (ish) was used to detect the relative expression of genes in the flourishing and retreating stages by real-time fluorescence quantitative PCR.The results showed that the five genes were specifically expressed in the skin. There was no significant difference in the expression of KAP6-1.2 and KAP8.1 between the cortex layer of the secondary hair follicle and the inner root sheath of the secondary hair follicle at the beginning of the prosperous period, and there was no significant difference in the expression of KAP6.2 in the secondary hair follicle at the beginning of the prosperous period.The expression of KAP7.1 and KAP8.2 in primary hair follicles was higher than that in primary hair follicles, but in retrogression stage, the expression of KAP7.1 and KAP8.2 was higher than that in primary hair follicles.It is suggested that the HGT KAPs gene family plays an important role in regulating the fiber diameter of Liaoning cashmere goat and is the gene encoding cashmere structural protein.
【作者单位】：辽宁师范大学生命科学学院辽宁省生物技术与分子药物研发重点实验室;
【基金】：国家自然科学基金(31172188) 辽宁省教育厅科学研究项目(L20144255) 辽宁省特聘教授资助计划(2014.1.1-2016.12.31)
【分类号】：S827.2

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