重组质粒pcDNA3-IL-2对鸡球虫疫苗的免疫增效作用

发布时间：2018-04-17 22:38

本文选题：pcDNA-IL-重组质粒 + 球虫疫苗　；参考：《中国兽医学报》2017年03期

【摘要】：为了确定重组质粒pcDNA3-IL-2对鸡球虫疫苗的免疫增效作用,将60只14日龄雏鸡随机分成5组,分别为对照组、球虫活疫苗免疫组、球虫活疫苗免疫加50、100、150μg/只重组质粒组。分组当天进行鸡球虫病四价活疫苗免疫,1,7d分别肌肉注射重组质粒pcDNA3-IL-2。至21d时,除第1组(对照组)外,每只鸡经口灌服E.tenella卵囊8×104个。对免疫后鸡的生长性能,外周血淋巴细胞转化率,血清IFN-γ、IL-2、IgG含量以及攻虫后的抗球虫指数进行测定。结果表明,pcDNA3-IL-2重组质粒对鸡球虫疫苗免疫增效的适宜剂量为100μg/只,与第2组比较,14、21、28d的外周血淋巴细胞转化率显著提高了26.54%、27.15%和24.19%(P0.05);血清IFN-γ含量显著提高了8.87%、14.56%和15.18%(P0.05);血清IL-2和IgG含量分别提高了3.40%、4.09%、3.95%和12.75%、9.73%、9.87%,胸腺、法氏囊与脾脏指数分别提高了4.91%、9.27%和2.46%(P0.05),ACI指数达180.71,提高了9.24%。
[Abstract]:In order to determine the immune effect of recombinant plasmid pcDNA3-IL-2 on chicken coccidiosis vaccine, 60 14-day-old chicks were randomly divided into five groups: control group, live coccidiosis vaccine immunization group, coccidiosis live vaccine immunization group and 50100150 渭 g / kg recombinant plasmid group.The recombinant plasmid pcDNA3-IL-2 was injected intramuscularly for 1 day and 7 days after immunization with live vaccine against coccidiosis.At the 21st day, except the first group (control group), each chicken was given 8 脳 104 E.tenella oocysts orally.The growth performance, lymphocyte transformation rate of peripheral blood, the content of serum IFN- 纬 -IL-2IgG and the anti-coccidiosis index of chickens after immunization were measured.The results showed that the optimal dose of pcDNA3-IL-2 recombinant plasmid for chicken coccidiosis vaccine immunization was 100 渭 g / mouse.Compared with the second group, the transformation rate of peripheral blood lymphocytes increased significantly by 26.54% and 24.19% respectively (P 0.05); the content of serum IFN- 纬 increased significantly by 8.87 ~ 14.56% and 15.18%; and the contents of serum IL-2 and IgG increased by 3.404.09 ~ 3.95% and 12.75 ~ 9.73 ~ 9.87%, respectively, in thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus, thymus.The index of bursa Fabricius and spleen increased by 4.91% and 2.46%, respectively, and the ACI index of P0.05 was 180.71, which increased 9.24%.
【作者单位】：河北科技师范学院河北省预防兽医重点实验室;
【基金】：河北省自然科学基金资助项目(C2014407043) 河北省蛋鸡产业技术体系创新团队资助项目(HBCT2013090203) 河北省教育厅重点资助项目(ZD2014071)
【分类号】：S858.31

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