鸭源致病性大肠杆菌的分离鉴定及系统发育分析

发布时间：2018-04-18 04:22

本文选题：禽致病性大肠杆菌 + 鸭　；参考：《中国兽医科学》2017年03期

【摘要】：为确定江苏省徐州市某养鸭场病死鸭的病原菌,从发病鸭的肝中分离到1株细菌并命名为JSE 4,根据形态特征、培养特性、生化试验、16S r R N A分子鉴定结果可确定该菌为大肠杆菌(E.coli)。对该分离菌进行致病型快速鉴定、动物致病试验、毒力基因检测、药敏试验、系统发育分析,结果显示,该分离株为禽致病性大肠杆菌(APEC),可引起试验组SPF雏鸭死亡,死亡率为16.7%。分离株含有14种毒力基因中的12种,29种常用抗菌药物中,该分离株仅对链霉素、呋喃妥因、亚胺培南、呋喃唑酮、头孢他啶、氨曲南这6种药物具有敏感性。该分离株与源自比利时水源、瑞士禽肉源和中国鸭源分离株的亲缘关系最近,与比利时水源、瑞士禽肉源分离株的同源性高达99.7%。研究表明,从江苏徐州周边养鸭场病死鸭中分离到1株多重耐药的禽致病性大肠杆菌。
[Abstract]:In order to determine the pathogenic bacteria of dead duck in a duck farm in Xuzhou City, Jiangsu Province, a strain of bacteria named JSE 4 was isolated from the liver of infected duck.The result of molecular identification of 16s r R na in biochemical test showed that the strain was E. coliformis.Rapid identification of pathogenic type, animal pathogenicity test, virulence gene test, drug sensitivity test and phylogenetic analysis showed that the isolated strain was avian pathogenic Escherichia coli and could cause death of SPF ducklings in the experimental group, with a mortality of 16.7.The isolates contained 12 or 29 common antimicrobials of 14 virulence genes. The isolates were only sensitive to streptomycin, furantoin, imipenem, furazolidone, ceftazidime and aztreonam.This isolate has the closest relationship with the water source of Belgium, Swiss poultry and Chinese duck, and has 99.7 homology with Belgian water source and Swiss poultry meat source isolate.The results showed that a multidrug resistant avian pathogenic Escherichia coli was isolated from the dead duck from the duck farm around Xuzhou Jiangsu province.
【作者单位】：牡丹江师范学院生命科学与技术学院;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室;东北农业大学生命科学学院;
【基金】：国家科技支撑计划项目(2015BA I07B02-02) 黑龙江省自然科学基金重点项目(ZD2016006)
【分类号】：S852.612

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