脂筏在牛呼吸道合胞体病毒感染MDBK细胞中的作用

发布时间：2018-04-18 22:41

本文选题：牛呼吸道合胞体病毒 + 脂筏　；参考：《中国兽医学报》2017年04期

【摘要】：为探讨脂筏在牛呼吸道合胞体病毒(bovine respiratory syncytial virus,BRSV)感染MDBK细胞中的作用,采用胆固醇去除药物MβCD处理MDBK细胞。结果显示,细胞膜胆固醇含量明显降低,同时也降低BRSV感染水平,补充外源胆固醇后,病毒感染力可部分恢复;表明细胞膜胆固醇的去除可以抑制BRSV的感染,BRSV的感染与细胞膜脂筏相关。进一步研究发现,胆固醇去除不会影响BRSV结合在靶细胞表面,但会抑制病毒进入靶细胞。在病毒感染后采用胆固醇去除药物MβCD处理MDBK细胞,对于病毒感染力的影响是轻微的。这些发现可有助于理解BRSV的感染机制,为研制安全、高效的疫苗和有效的治疗药物提供理论依据。
[Abstract]:To investigate the role of lipid rafts in the infection of bovine respiratory syncytial virus (BRSVV) with bovine respiratory syncytial virus, MDBK cells were treated with cholesterol removal drug M 尾 CD.The results showed that the content of plasma membrane cholesterol was significantly decreased and the level of BRSV infection was also decreased. After supplementation of exogenous cholesterol, the viral infectivity could be partially recovered.The results showed that the removal of membrane cholesterol could inhibit the infection of BRSV.Further studies showed that cholesterol removal did not affect the binding of BRSV to target cells, but inhibited virus entry into target cells.After virus infection, MDBK cells were treated with cholesterol removal drug M 尾 CD, and the effect on viral infectivity was slight.These findings may be helpful to understand the mechanism of BRSV infection and provide theoretical basis for the development of safe, efficient vaccines and effective therapeutic drugs.
【作者单位】：黑龙江八一农垦大学生命科学技术学院;黑龙江八一农垦大学动物科技学院;
【基金】：黑龙江八一农垦大学校内培育课题资助项目(XZR2014-14) 黑龙江省教育厅课题资助项目(12521372) 国家科技计划课题资助项目(2012BAD12B05-2)
【分类号】：S858.23

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