不同电融合参数对猪手工克隆重构胚胎发育效果的影响

发布时间：2018-04-19 01:08

本文选题：猪 + 电融合参数　；参考：《湖北农业科学》2016年21期

【摘要】：为探讨不同融合参数对猪手工克隆(HMC)重构胚发育效果的影响。本试验比较了胞质与胞质之间不同黏合方式以及不同的电融合参数对融合效率的影响,以及融合参数对HMC重构胚胎发育的影响。结果表明,卵-供体-卵的黏合方式有利于提高胞质的融合率;融合参数3(2 k V/cm,80μs,1次)和参数4(2.2 k V/cm,80μs,1次)的对胞质的融合率显著高于其他参数,两个参数对HMC重构胚卵裂率的影响差异不显著,而参数3的囊胚率以及囊胚细胞总数显著高于参数4。参数3更适合用于HMC重构胚的融合,并能提高重构胚的囊胚发育率以及囊胚的细胞个数。
[Abstract]:To investigate the effect of different fusion parameters on the development of HMC-based reconstructed embryos.The effects of different adhesion modes between cytoplasm and cytoplasm and different electrofusion parameters on fusion efficiency and the effects of fusion parameters on the development of HMC reconstructed embryos were compared.The results showed that the oval-donor-egg bonding mode was beneficial to the improvement of the cytoplasmic fusion rate, and the fusion rate of the fusion parameters (3 ~ 2 kV / cm ~ (-1) and 42.2 kV / cm ~ (80 渭 s / m ~ (-1))) was significantly higher than that of the other parameters.There was no significant difference between the two parameters on cleavage rate of HMC reconstructed embryos, but the blastocyst rate and the total number of blastocysts in parameter 3 were significantly higher than those in parameter 4.Parameter 3 is more suitable for the fusion of HMC reconstructed embryos and can improve the blastocyst development rate and the number of blastocysts.
【作者单位】：广西壮族自治区畜牧研究所/广西家畜遗传改良重点实验室;广西壮族自治区水牛研究所;
【基金】：国家自然科学基金项目(31360544) 广西畜牧研究所基本科研业务费项目(桂牧研科2015-02)
【分类号】：S828

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