石蜡切片H.E.染色后废乙醇的回收技术

发布时间：2018-04-19 22:13

  本文选题：石蜡切片 + H.E.染色　； 参考：《黑龙江畜牧兽医》2017年08期

【摘要】：苏木精-伊红染色法(hematoxylin-eosin staining)简称H.E.染色法,广泛应用于科学试验中。常规的H.E.染色中需要用到大量的梯度乙醇溶液,但这些乙醇溶液使用几次后就成了混悬液,无法重复使用,不但使试验成本增高,造成很大浪费,而且还污染了环境。笔者通过长期的实践总结出了一种用于石蜡切片H.E.染色后废乙醇的回收方法,且经试验证明通过该方法回收的梯度乙醇与新乙醇制作出来的组织病理切片没有任何的区别,可使废乙醇变废为宝,延长了乙醇的使用寿命,提高了乙醇的利用率,为实验室节约了实验经费的同时还减少了环境的污染。
[Abstract]:Hematoxylin-eosin stenting (H. E. Staining method is widely used in scientific experiments. Conventional H. E. A large number of gradient ethanol solutions are used in dyeing, but these ethanol solutions become suspensions after several times, which can not be reused, which not only increases the test cost, but also pollutes the environment. In this paper, a new method for paraffin slice H. E. The method of recovering waste ethanol after dyeing is proved by experiment that there is no difference between the gradient ethanol recovered by this method and the tissue pathological section made by new ethanol, which can turn waste ethanol into treasure and prolong the service life of ethanol. The utilization rate of ethanol was improved, and the experiment cost was saved, and the environmental pollution was reduced at the same time.
【作者单位】： 塔里木大学动物科学学院;
【基金】：国家级动物医学实验教学示范中心建设项目 塔里木大学动物医学专业综合改革试点项目;塔里木大学高等教育教学研究重点项目(TDGJ1310)
【分类号】：S852.31
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本文编号：1774963