lncLER在卢氏绿壳蛋鸡肝脏中表达调控的研究

发布时间：2018-04-20 03:11

本文选题：长链非编码RNA + 卢氏绿壳蛋鸡　；参考：《中国畜牧兽医》2017年04期

【摘要】：长链非编码RNA(lncRNA)在许多生物学过程中发挥着重要的调节作用。河南农业大学家禽种质资源创新工程研究中心通过对产蛋前期(20周龄)和产蛋高峰期(30周龄)卢氏绿壳蛋母鸡肝脏组织的转录组分析,筛选出了一个在产蛋高峰期母鸡肝脏中表达水平显著上调的lncRNA(lncLER)。本研究利用半定量PCR(RT-PCR)、实时荧光定量PCR分析了lncLER在卢氏绿壳蛋母鸡不同发育时期和不同组织中的表达规律,并从个体和细胞水平研究了体内、体外雌激素处理对lncLER表达的影响。结果表明,lncLER在卢氏绿壳蛋母鸡各组织中均有不同程度的表达,且在产蛋高峰期肝脏中的表达量较高;雌激素在个体和细胞水平可显著上调lncLER的表达。本试验初步阐明了lncLER基因在卢氏绿壳蛋母鸡中的表达调控规律,为进一步研究lncLER在鸡产蛋过程中的生物学功能奠定了基础。
[Abstract]:Long chain noncoding RNAs (LNRNAs) play an important regulatory role in many biological processes. The Research Center for innovative Engineering of Poultry Germplasm Resources of Henan Agricultural University analyzed the liver tissue of Lu's green egg hen by 20 weeks of early laying and 30 weeks of peak egg laying. A significantly up-regulated expression level of lncRNA-lerr in the liver of laying peak hens was selected. In this study, the expression of lncLER in different tissues and development stages of Lushi green egg hens was analyzed by using semi-quantitative PCR RT PCR and real-time fluorescence quantitative PCR, and the effects of estrogen treatment on lncLER expression in vivo and in vitro were studied at the individual and cell levels. The results showed that the expression of lncLER in all tissues of Lushi green egg hens was higher than that in the liver during the peak period of egg laying, and estrogen could upregulate the expression of lncLER at individual and cell levels. The regulation of lncLER gene expression in Lushi green egg hens was preliminarily elucidated in this study, which laid a foundation for further study on the biological function of lncLER during laying.
【作者单位】：石河子大学动物科技学院;河南农业大学牧医工程学院;河南省家禽种质资源创新工程研究中心;
【基金】：河南省国际合作项目(162102410030) 地方鸡种质资源保护与利用教育部创新团队发展计划(IRT16R23) 国家蛋鸡产业技术体系遗传育种岗位专项资金(CARS-41-K04)
【分类号】：S831

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