禽波氏杆菌鸡胚强毒株对雏鸡的致病性

发布时间：2018-04-20 03:12

本文选题：禽波氏杆菌 + 鸡胚强毒株　；参考：《中国兽医学报》2017年11期

【摘要】：以从孵化场将出壳鸡胚中分离到的鸡胚强毒株禽波氏杆菌LL09作为试验菌株,分别经鼻腔、眼睛和腹腔3种感染方式和1,7,14,21d不同日龄感染海蓝褐蛋雏鸡,检测其致病过程、病理变化,以及LL09在不同感染方式和不同感染日龄对雏鸡的生长抑制和NDV灭活苗免疫后HI抗体水平的影响差异。结果发现:LL09能引起雏鸡眼睛分泌物增多,咳嗽等呼吸道症状,且能入侵内脏组织,导致气管黏膜纤毛细胞脱落,肺出血,心肌细胞、肝细胞、脾细胞、肾小管上皮细胞等变性坏死等病理变化。3种感染方式中以腹腔感染危害最为严重,入侵内脏组织的比例最高,对雏鸡的生长抑制作用最强,对NDV免疫后HI抗体水平的影响最大,其次是经鼻腔感染组和经眼睛感染组。LL09分别于不同日龄感染雏鸡结果发现,感染日龄与LL09对雏鸡的危害直接相关,越早期感染,对雏鸡的体质量抑制越严重,对NDV免疫后的HI抗体水平的影响越大,内脏组织的感染几率越高。尤其是1d和7d感染组危害比较严重,而21d感染雏鸡症状不明显,且未在除气管和肺脏以外的内脏组织中检测到LL09的感染。对禽波氏杆菌LL09鸡胚强毒株的致病性检测结果表明,LL09作为鸡胚分离株,同样可以导致禽类的呼吸道感染,且能入侵内脏组织,以腹腔感染和1周内感染的危害最为严重。
[Abstract]:The virulent strain LL09 of chicken embryo isolated from hatchery was used as the experimental strain, infected with sea blue brown egg chicks at different ages of 21 days, through nasal cavity, eyes and abdominal cavity, respectively, and the pathogenicity was detected. Pathological changes and the effect of LL09 on growth inhibition and HI antibody level of chickens after NDV inactivated vaccine immunization with different infection modes and different infection days. The results showed that WLL09 could induce respiratory symptoms such as increased eye secretion, cough and other respiratory symptoms in chicks, and could invade visceral tissue, resulting in exfoliation of cilia cells of trachea mucosa, pulmonary hemorrhage, cardiomyocytes, hepatocytes and spleen cells. Among the pathological changes of renal tubular epithelial cells such as isomatosis and necrosis, intraperitoneal infection was the most serious, the proportion of invading visceral tissue was the highest, the inhibitory effect on the growth of chicks was the strongest, and the HI antibody level after NDV immunization was the biggest. The results showed that the age of infection was directly related to the harm of LL09 to the chicks. The earlier the infection was, the more serious the inhibition of the body mass of the chicks was. The higher the effect on HI antibody level after NDV immunization, the higher the infection rate of visceral tissue. Especially in the 1st and 7th day groups, the damage was more serious, but the symptoms of 21 days infection were not obvious, and the infection of LL09 was not detected in the visceral tissues except trachea and lung. The pathogenicity of virulent strain of LL09 chicken embryo showed that LL09, as a isolate of chicken embryo, could also cause respiratory tract infection and invasion of visceral tissue, especially in abdominal cavity and within one week.
【作者单位】：山东农业大学动物科技学院;山东省动物疫病预防与控制中心;
【基金】：国家自然科学基金资助项目(31602067,31772778) 山东省自然科学基金资助项目(ZR2014CM044) 山东省“双一流”奖补资金资助项目
【分类号】：S858.31

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