颈静脉灌注油脂对泌乳奶牛乳腺氨基酸摄取率的影响

发布时间：2018-04-20 17:42

本文选题：泌乳奶牛 + 灌注　；参考：《中国畜牧杂志》2017年01期

【摘要】：本试验旨在研究颈静脉灌注不同组合的油脂对泌乳奶牛乳腺氨基酸摄取率的影响。试验选用体况良好、体重相近,泌乳早期,产奶量25 kg/d左右装有颈静脉插管的荷斯坦奶牛8头,进行4×4拉丁方试验。预试期14 d,每期的试验期12 d,包括5 d灌注期和7 d恢复期。分别从颈静脉灌注处理组(A)橄榄油+红花籽油、处理组(B)橄榄油+胡麻油、处理组(C)红花籽油+胡麻油和处理组(D)橄榄油+红花籽油+胡麻油。在灌注期的最后一天,采集乳样、尾动脉血和乳静脉血。乳样用于测定乳成分,血样用于测定血中氨基酸含量并计算乳腺氨基酸摄取效率。结果表明:当给泌乳奶牛的颈静脉灌注不同组合的油脂后对尾动脉血中Cys和Val的含量差异显著(P0.05),Arg和BCAA的含量有差异显著的趋势(P=0.12;P=0.08),其余各个AA含量均差异不显著(P0.05);在动静脉差和摄取效率上,单一AA、TAA、EAA、NEAA、BCAA、LAA均差异不显著(P0.05);但B组的动脉氨基酸浓度、氨基酸的动静脉差以及氨基酸的摄取效率均高于其他处理组,但差异不显著(P0.05)。泌乳奶牛静脉灌注橄榄油和胡麻油所提供的长链不饱和脂肪酸(C18:1和C18:3)组合对乳腺氨基酸的摄取有一定的促进作用,但表现为低乳蛋白含量。
[Abstract]:The purpose of this study was to study the effect of jugular vein infusion of different combinations of oils on breast amino acid uptake in lactating cows. In the experiment, 8 Holstein cows with good body condition, similar body weight, early lactation and 25 kg/d milk yield were used for 4 脳 4 Latin square test. The pretest period was 14 days and the trial period was 12 days, including 5 d perfusion period and 7 d recovery period. Olive oil safflower seed oil, treatment group B) olive oil flax oil, treatment group C) safflower seed oil flax oil and treatment group D) olive oil safflower seed oil flax oil were perfused from jugular vein. On the last day of the perfusion period, milk samples, caudal blood and mammary vein blood were collected. Milk samples are used to determine milk ingredients, blood samples are used to determine amino acids in blood and breast amino acid uptake efficiency is calculated. The results showed that there was significant difference in the contents of Cys and Val in caudal blood after the jugular vein was infused with different combinations of grease in lactating cows. There was a significant difference in the contents of Cys and BCAA in caudal blood. In terms of arteriovenous difference and uptake efficiency, There was no significant difference in the concentration of arterial amino acids, the arteriovenous difference of amino acids and the uptake efficiency of amino acids in group B, but the difference was not significant (P 0.05). The combination of long chain unsaturated fatty acid C18: 1 and C18: 3 provided by intravenous infusion of olive oil and flax oil in lactating cows promoted breast amino acid uptake to some extent, but showed low milk protein content.
【作者单位】：内蒙古农业大学动物科学学院;
【基金】：国家奶业973计划(2011CB100803)
【分类号】：S823.5

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