nisin及大黄复方对子宫内膜炎大鼠血清中MMP-2和MMP-9活性的影响

发布时间：2018-04-22 02:31

本文选题：nisin + 大黄复方　；参考：《中国农业大学学报》2017年06期

【摘要】：为研究乳酸链球菌素(nisin)及大黄复方对患大鼠子宫内膜炎血清中基质金属蛋白酶-2、基质金属蛋白酶-9(MMP-2、MMP-9)的作用,构建大鼠子宫内膜炎模型,将280只SD大鼠随机分为nisin组、低、中、高剂量大黄复方组、阳性对照组、生理盐水组以及模型组,建子宫内膜炎模型前0h和建模后24、48、72h,用ELISA试剂盒检测各试验组中基质金属蛋白酶(MMPs)和基质金属蛋白抑制物(TIMPs)的变化情况来确定治疗效果。结果表明:1)在0~72h之间,模型组中,MMP-9和TIMP-1的质量浓度显著低于生理盐水组(P0.05),且MMP-2和TIMP-2的质量浓度逐渐降低;2)nisin和高剂量大黄复方组中MMP-9、TIMP-1、MMP-2和TIMP-2的质量浓度均显著高于生理盐水组(P0.05),其中以nisin组和高剂量大黄复方组效果最佳。由此可见,nisin和高剂量大黄复方能显著提高子宫内膜炎大鼠血清中TIMP-1和TIMP-2的表达,抑制MMP-9和MMP-2的活性而发挥调控子宫内生理机能的作用,表明nisin、中、高剂量大黄复方对MMP-9、TIMP-1和MMP-2的质量浓度具有正调控作用。
[Abstract]:In order to study the effects of nisin) and rhubarb compound on matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-2MMP-9) in serum of endometritis rats, 280 SD rats were randomly divided into nisin group, low group and middle group. High dose rhubarb compound group, positive control group, normal saline group and model group, The changes of matrix metalloproteinase (MMPs) and matrix metalloproteinase inhibitor (TIMPs) were detected by ELISA kit in order to determine the therapeutic effect. The results show that 1) is between 0 and 72 hours. The mass concentrations of MMP-9 and TIMP-1 in the model group were significantly lower than those in the saline group (P 0.05), and the mass concentrations of MMP-2 and TIMP-2 decreased gradually, and the mass concentrations of MMP-9 TIMP-1MMP-2 and TIMP-2 in the high dose rhubarb compound group were significantly higher than those in the saline group, especially in the nisin group. And high dose rhubarb compound group is the best. It can be seen that nisin and high dose rhubarb compound can significantly increase the expression of TIMP-1 and TIMP-2 in the serum of endometritis rats, inhibit the activity of MMP-9 and MMP-2, and play a role in regulating the physiological function of uterus. High dose rhubarb compound has positive effect on the mass concentration of MMP-9 and TIMP-1 and MMP-2.
【作者单位】：内蒙古农业大学兽医学院;内蒙古农业大学动物科学学院;
【基金】：国家自然科学基金项目(31260590) 国家科技支撑项目(2015BAD29B06)
【分类号】：S857.22

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