猪巨细胞病毒福建株DPOL基因的克隆及序列分析

发布时间：2018-04-22 06:10

本文选题：猪巨细胞病毒 + DPOL基因　；参考：《中国畜牧兽医》2017年02期

【摘要】：为了明确猪巨细胞病毒(porcine cytomegalovirus,PCMV)福建株(PCMV-FJ01株)DPOL基因特征,本研究根据GenBank数据库中PCMV DPOL基因序列特征,利用引物设计软件Oligo 7.0设计针对DPOL基因的引物,以PCMV-FJ01株核酸DNA为模板,对其进行分段PCR扩增。将分段扩增的产物经胶回收试剂盒回收后进行克隆测序,对测序结果进行拼接后获得PCMV-FJ01株DPOL基因序列,并进行生物信息学分析。结果表明,PCMV-FJ01株DPOL基因全长为3 021bp,编码1 006个氨基酸;其与GenBank中的PCMV分离株DPOL基因核苷酸和氨基酸同源性分别在98.7%和99.1%以上。遗传进化分析发现,相对于巨细胞病毒属其他种代表株,PCMV分离株DPOL基因在遗传进化上和玫瑰疱病毒属代表株更近。建议根据PCMV DPOL基因遗传进化特征,将其划归为玫瑰疱病毒属的一个病毒新种——猪玫瑰疹病毒(porcine roseolovirus)。
[Abstract]:In order to identify the characteristics of PCMV DPOL gene of porcine cytomegalovirus (PCMV) Fujian strain PCMV-FJ01 strain, a primer design software Oligo 7.0 was used to design primers for DPOL gene based on the sequence characteristics of PCMV DPOL gene in GenBank database. The nucleic acid DNA of PCMV-FJ01 strain was used as template. It was amplified by segmental PCR. The fragment amplified product was recovered by gel recovery kit and cloned and sequenced. The sequence of DPOL gene of PCMV-FJ01 strain was sequenced and analyzed by bioinformatics. The results showed that the total length of DPOL gene of PCMV-FJ01 strain was 3 021 BP, encoding 1 006 amino acids, and the nucleotide and amino acid homology between PCMV-FJ01 strain and PCMV isolate was 98.7% and 99.1%, respectively. Genetic evolution analysis showed that the DPOL gene was closer to the representative strain of Rosa in genetic evolution compared with other representative strains of cytomegalovirus. According to the genetic and evolutionary characteristics of PCMV DPOL gene, it is suggested that it be classified as porcine roseolovirusa, a new virus species of Rosa genus.
【作者单位】：福建省农业科学院畜牧兽医研究所福建省畜禽疫病防治工程技术研究中心;福安市动物疾病预防控制中心;
【基金】：福建省属公益类科研院所基本科研专项(2014R1023-13、2014R1023-16)
【分类号】：S852.651

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