猪流行性腹泻病毒分离鉴定及其灭活疫苗免疫保护效果研究

发布时间：2018-04-22 15:39

本文选题：猪流行性腹泻病毒 + 分离鉴定　；参考：《动物医学进展》2017年01期

【摘要】：为筛选能用于猪流行性腹泻病毒(PEDV)疫苗研发的流行毒株,收集PEDV阳性病料,以Vero细胞进行病毒分离试验,并对分离毒株进行外源病毒检测、病毒培养特性研究、仔猪毒力试验及免疫效力试验等。9份PEDV阳性病料共分离到3株病毒,分别命名为PEDV-SC12、PEDV-JS12、PEDV-JX12。其中PEDV-SC12株存在支原体污染;PEDV-JS12株与PEDV-JX12株均能被PEDV特异性阳性血清中和;PEDV-JS12株能在Vero细胞上增殖并产生细胞病变,但适应细胞45代以后病毒培养效价仍然偏低;PEDV-JX12滴度能维持在106.0 TCID50/mL以上。PEDV-JX12株毒力试验显示,该分离株能够通过人工感染复制出腹泻病例,并能从发病动物体内检测到感染的病毒。免疫攻毒保护试验显示,以PEDV-JX12株制备的灭活疫苗免疫母猪,对所产仔猪攻毒后免疫组6.25%(1/16)发病,对照组100%(8/8)发病。说明PEDV-JX12株能够适应细胞培养,免疫接种母猪能给仔猪提供有效保护,可以用于PEDV流行毒株的疫苗研发。
[Abstract]:In order to screen the epidemic strains which can be used in the research and development of porcine epidemic diarrhea virus (PEV) vaccine, PEDV positive venereal materials were collected, virus isolation tests were carried out with Vero cells, and the virus culture characteristics were studied. Three strains of virulence test and immune efficacy test of PEDV positive venereal diseases were isolated from piglets and named PEDV-SC12 PEDV-JX12. Both PEDV-SC12 strain and PEDV-JS12 strain could proliferate and cause cytopathic changes in Vero cells by PEDV specific positive serum. However, the virus titer of PEDV-JX12 was still low after 45 passage adaptation. The virulence test of PEDV-JX12 strain above 106.0 TCID50/mL showed that the isolated strain could replicate diarrhea cases by artificial infection, and the infected virus could be detected from the infected animals. The immunoprotection test showed that the inactivated vaccine prepared by PEDV-JX12 strain was used to immunize sows with 6.25 / 1 / 16 of the vaccinated piglets, and the control group was 100 / 8 / 8). The results showed that PEDV-JX12 strain could adapt to cell culture, immunized sows could provide effective protection for piglets, and could be used for vaccine development of PEDV epidemic strains.
【作者单位】：成都天邦生物制品有限公司;
【分类号】：S858.28

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