低温脱脂大豆粕的低丰度蛋白提取和高丰度蛋白去除条件的优化（英文）

发布时间：2018-04-23 22:01

本文选题：蛋白提取 + 高丰度蛋白　；参考：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年10期

【摘要】：目的:优化低温脱脂大豆粕中的低丰度蛋白提取和高丰度蛋白去除条件,为进一步探讨大豆低丰度蛋白对动物的生理功能影响提供试验材料。创新点:结合提取时间和异丙醇浓度,将超声波(超声时间、功率)用于辅助异丙醇去除大豆高丰度蛋白和富集低丰度蛋白的研究。方法:在单因素试验基础上,设计了超声时间、超声功率、提取时间和异丙醇浓度等四因素三水平的正交试验。并通过测定提取液中的蛋白浓度和通过聚丙烯酰胺凝胶电泳(SDS-PAGE)、质谱分析(MS)等蛋白质组学手段鉴定提取液或丙酮沉淀物中的蛋白质含量,实现了大豆高丰度蛋白去除和低丰度蛋白提取条件的优化。结论:50%异丙醇,350 W超声15 min,提取1 h是大豆低丰度蛋白提取的最佳条件。提取物含多种低丰度蛋白(油质蛋白、大豆未知蛋白、磷酸酯D和无特征大豆蛋白),且无大豆高丰度蛋白(大豆球蛋白和β-伴大豆球蛋白)。
[Abstract]:Objective: to optimize the conditions of low abundance protein extraction and high abundance protein removal from low temperature defatted soybean meal, and to provide experimental materials for further study on the effect of soybean low abundance protein on physiological function of animals. Innovation: combined with extraction time and isopropanol concentration, ultrasonic wave (ultrasonic time, power) was used to assist isopropanol to remove high abundance soybean protein and enrich low abundance protein. Methods: on the basis of single factor experiment, orthogonal experiments were designed with four factors: ultrasonic time, ultrasonic power, extraction time and isopropanol concentration. The protein concentration in the extract was determined and the protein content in the extract or acetone precipitate was identified by means of proteomics, such as polyacrylamide gel electrophoresis, SDS-PAGEG, MS, etc. The extraction conditions of soybean high abundance protein and low abundance protein were optimized. Conclusion the optimum extraction condition of soybean protein with low abundance was 50% isopropanol 350W for 15 min and 1 hour for extraction. The extract contains a variety of low abundance proteins (oil protein, soybean unknown protein, phosphate D and non-characteristic soybean protein), and no soybean high abundance protein (soybean globulin and 尾 -concomitant soybean globulin).
【作者单位】： College
【基金】：Project supported by the China Agriculture Research System(No.CARS-36) the National Natural Science Foundation of China(No.31572430)
【分类号】：S816.4

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