油脂类型对肉鸡不同组织甘油醛-3-磷酸脱氢酶基因相对表达量的影响

发布时间：2018-04-23 22:28

本文选题：肉鸡 + 油脂　；参考：《动物营养学报》2017年05期

【摘要】：本试验旨在探究油脂类型对肉鸡不同组织甘油醛-3-磷酸脱氢酶(GAPDH)基因相对表达量的影响。试验选用240只1日龄的科宝肉鸡母雏,随机分为8个组(4个单一油脂组,分别在饲粮中添加5.00%亚麻油、玉米油、芝麻油和猪油;4个混合油脂组,分别在饲粮中添加2.50%猪油+2.50%玉米油、2.50%猪油+2.50%芝麻油、2.50%亚麻油+2.50%玉米油和2.50%亚麻油+2.50%芝麻油),每组6个重复,每个重复5只鸡。试验期42 d。结果表明:1)组织及油脂类型与组织的交互作用对42日龄肉鸡组织GAPDH基因的相对表达量影响显著(P0.05),油脂类型对42日龄肉鸡组织GAPDH基因的相对表达量的影响不显著(P0.05)。42日龄肉鸡胸肌GAPDH基因的相对表达量显著高于肝脏和腹脂(P0.05),是肝脏的37.50~89.50倍,是腹脂的129.54~190.64倍,而42日龄肉鸡的GAPDH基因的相对表达量在肝脏与腹脂之间差异不显著(P0.05);玉米油组胸肌中GAPDH基因的相对表达量显著高于猪油组(P0.05)。2)21日龄肉鸡肝脏GAPDH基因的相对表达量显著或极显著高于42日龄(P0.05或P0.01)。3)油脂组合及油脂组合与日龄的交互作用对肉鸡肝脏GAPDH基因相对表达量的影响均不显著(P0.05),但日龄对肉鸡肝脏GAPDH基因相对表达量的影响显著(P0.05)。由此可见,油脂类型对肉鸡GAPDH基因的相对表达量的影响呈现组织间的差异,玉米油可提高胸肌GAPDH基因的相对表达量。42日龄肉鸡胸肌GAPDH基因的相对表达量显著高于肝脏和腹脂,21日龄肉鸡肝脏GAPDH基因的相对表达量显著高于42日龄
[Abstract]:The purpose of this study was to investigate the effect of oil type on the relative expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene in different tissues of broilers. 240 1-day-old Kebao broilers were randomly divided into 8 groups (4 single fat groups, supplemented with 5.00% linseed oil, corn oil, sesame oil and lard, respectively), 4 mixed fat groups, and 4 mixed fat groups, respectively, in which 5% linseed oil, corn oil, sesame oil and lard were added to the diet. 2.50% lard 2.50% sesame oil 2.50% sesame oil 2.50% linseed oil and 2.50% linseed oil 2.50% sesame oil were added to the diet. The experimental period was 42 days. The results showed that the relative expression of GAPDH gene in 42-day-old broiler tissues was significantly affected by the interaction between tissue and lipid type. The effect of lipid type on the relative expression of GAPDH gene in 42-day-old broiler tissues was not significant. The relative expression of GAPDH gene in breast muscle of day-old broilers was significantly higher than that in liver and abdominal fat (P 0.05), and was 89.50 times higher than that in liver. It's 129.54 and 190.64 times as much as abdominal fat. However, there was no significant difference in the relative expression of GAPDH gene between liver and abdominal fat in 42-day-old broilers, and the relative expression of GAPDH gene in pectoralis of corn oil group was significantly higher than that of lard group P0.05. 221 days old broilers. The effects of lipid combination and the interaction between lipid combination and day old on the relative expression of GAPDH gene in liver of broilers were not significant, but the effect of age on the relative expression of GAPDH gene in liver of broilers was significantly higher than that of control group (P0.05 or P0.01 路3), but the effect of age on the relative expression of GAPDH gene in liver of broilers was significant (P 0.05). It can be seen that the effect of oil type on the relative expression of GAPDH gene in broilers shows the difference between tissues. Corn oil increased the relative expression of GAPDH gene in pectoralis muscle. The relative expression of GAPDH gene in breast muscle of 42-day-old broiler was significantly higher than that of liver and 21-day-old broiler liver. It was significantly higher than that of 42-day-old broiler.
【作者单位】：河南农业大学饲料营养河南省工程实验室河南农业大学国家家养动物种质资源平台;
【基金】：国家自然科学基金(31272434)
【分类号】：S831.5

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