2015-2016年广东省小鹅瘟病毒分子流行病学调查

发布时间：2018-04-24 08:42

本文选题：小鹅瘟病毒 + 分子流行病学　；参考：《仲恺农业工程学院》2017年硕士论文

【摘要】：小鹅瘟是由小鹅瘟病毒(Goose Parvovirus,GPV)引起3~20日龄雏鹅和雏番鸭的一种急性或亚急性败血性传染病,具有传播快、发病率高和死亡率高等特点,给养鹅业造成了重大损失。为研究小鹅瘟在广东省的流行情况,在2015年9月至2016年12月,从广东省各地采集到有疑似小鹅瘟症状的鹅肝脏与肠组织42份,共分离到7株GPV,分别是从粤北地区清远市分离到的QY1和QY2,从珠三角地区广州市分离到的GZ1和GZ2,从粤西地区阳江市分离到YJ1和YJ2,从粤西地区茂名市分离到的MM。对这7株GPV全基因进行测序,并对其序列、遗传进化和糖基化位点进行分析。结果显示如下:在全基因分析中,7株GPV的全长都为5050 bp,它们之间的同源性为99.8%~99.9%,在进化树中形成一个独立的新分支。7株GPV与GenBank上已发表的全基因序列同源性为93.4%~98.9%,与重庆毒株RC16亲缘关系最近,同源性为98.8%~98.9%;与标准株B同源性为98.2%~98.3%。对NS进行分析发现,7株GPV的NS1和NS2分别长为1884 bp和456 bp,分别可翻译成628和152个氨基酸。7株GPV的NS1的同源性为99.7%~100%,而NS2的同源性为100%。NS1只有个别碱基发生突变并引起氨基酸的变化,而NS2没有发生碱基的突变。7株GPV在NS1进化树中形成一个独立的新分支,与台湾毒株06-0329、82-0321以及重庆毒株RC16亲缘关系最为接近,同源性为99.1%~99.7%;与标准株B同源性为99.0%~99.2%。在NS2进化树中形成一个独立的新分支,与台湾毒株上海毒株SHFX1201和重庆毒株RC16亲缘关系最为接近,同源性为99.1%~99.6%;与标准株B同源性为98.7%。7株GPV的NS有着4个相同的糖基化位点,分别为150-152NKT、225-227NYS、360-362NWT、433-435NST,而GZ2比对比其他毒株多了330-332NAT糖基化位点。对VP进行分析发现,7株GPV的VP1、VP2和VP3分别长为2199 bp、1764 bp和1605 bp,分别可翻译成733、588和535个氨基酸。VP1、VP2和VP3同源性分别为99.6%~100%、99.6%~99.9%和99.6%~99.9%。7株GPV在VP1进化树中形成一个独立的新分支,与广东毒株GDFSh以及重庆毒株RC16亲缘关系最为接近,同源性为97.7%~98.2%;与标准株B同源性为97.4%~97.5%。在VP2进化树中形成一个独立的新分支,与标准株B同源性为97.8%~97.9%。在VP3进化树中与江苏毒株JSXZ-4和安徽毒株AH-1形成一个独立的新分支,亲缘关系最近,同源性为98.8%~99.8%;与标准株B同源性为97.9%~98.0%。7株GPV的VP有着5个相同的糖基化位点,分别为219-221NAS、331-333NLT、582-584NTT、700-702NFS、712-714NET。对ITR进行分析发现,7株GPV的ITR都长为416 bp,它们间的同源性为99.8%~100%。7株GPV在进化树中形成一个独立的新分支,与重庆毒株RC16和台湾毒株06-0329亲缘关系最近,同源性为97.0%~98.9%;与标准株B同源性为88.8%。本研究通过对从广东分离到的7株GPV基因序列、遗传进化和糖基化位点进行分析,旨在了解小鹅瘟病毒在广东省的进化方向和变异程度,为广东小鹅瘟的防控提供数据依据。
[Abstract]:Gosling plague is an acute or subacute septic infectious disease caused by Goose Parvovirus (GPVV) of Gosling Plague virus (Goose Parvovirus). It is an acute or subacute septic infectious disease of goslings and young muscovy ducks at the age of 20 days. It has the characteristics of fast transmission, high incidence and high mortality, and has caused great losses to the goose industry. In order to study the prevalence of Gosling Plague in Guangdong Province, 42 goose liver and intestinal tissues with suspected symptoms of Gosling plague were collected from various parts of Guangdong Province from September 2015 to December 2016. Seven strains of GPVs were isolated from Qingyuan city in northern Guangdong, GZ1 and GZ2 from Guangzhou city in Pearl River Delta, YJ1 and YJ2-2 from Yangjiang city in western Guangdong, and MM. from Maoming city in western Guangdong. The whole GPV genes of the 7 strains were sequenced and their sequences, genetic evolution and glycosylation sites were analyzed. The results are as follows: in the whole gene analysis, the total length of GPV of the 7 strains is 5050 BP, the homology between them is 99.8 and 99.9. The homology between GPV and the published whole gene sequence published on GenBank is 93.4% 98.9, which is similar to that of the whole gene sequence published on GenBank, and the homology between them is 99.8% and 99.9% respectively. The RC16 strains of Chongqing are closely related to each other. The homology was 98.8% and 98.2% with the standard strain B. It was found that the NS1 and NS2 of GPV were 1884 BP and 456bp, respectively. The homology of NS1 in GPV of 628 and 152 amino acid 7 strains was 99.7%, while the homology of NS2 was that only a few base pairs of 100%.NS1 mutated and caused amino acid changes. However, GPV, which had no base mutation in NS2, formed an independent new branch in the NS1 evolutionary tree, most closely related to Taiwan strain 06-0329 (82-0321) and Chongqing strain (RC16), with the homology of 99.1% and 99.7%, and 99.0% and 99.2% with standard strain B. An independent branch was formed in the NS2 phylogenetic tree, most closely related to the Taiwan strain Shanghai strain SHFX1201 and Chongqing strain RC16 strain, with a homology of 99.1 and 99.6, and had four identical glycosylation sites with standard strain B homology of 98.7.7 strain GPV. The results were as follows: 150-152 NKTX 225-227NYSU 360-362NWTT 433-435NST, and GZ2 had more 330-332NAT glycosylation sites than other strains. The VP2 and VP3 of GPV were 2199 BP and 1605 BP, respectively, which could be translated into 733588 and 535 amino acids, respectively. The homology of VP2 and VP3 were 99.699% and 99.699.9.7% and 99.699.9.7%, respectively. The genetic relationship with Guangdong strain GDFSh and Chongqing strain RC16 is the closest, the homology is 97.798. 2, and the homology with standard strain B is 97.4 and 97.5. An independent branch was formed in the VP2 phylogenetic tree, and the homology with the standard strain B was 97.8% and 97.9%. An independent branch was formed in the VP3 phylogenetic tree with Jiangsu strain JSXZ-4 and Anhui strain AH-1, with the closest genetic relationship, the homology was 98.8% and 99.8%, and the VP of the standard strain B with 97.9% homology and 98.0.7% had five identical glycosylation sites, respectively, which were 219-221 NAS331-333NLTT 582-584NTT 700-702NFS 712-714NET. The results of ITR analysis showed that the ITR length of 7 GPV strains was 416bp. the homology among them was 99.8kW and 100.7 strains formed an independent new branch in the evolutionary tree, which was closely related to the Chongqing strain RC16 and Taiwan strain 06-0329. The homology was 97.0 and 98.9, and the homology with standard strain B was 88.8. In this study, the sequence, genetic evolution and glycosylation sites of 7 strains of GPV gene isolated from Guangdong province were analyzed in order to understand the evolutionary direction and variation of Gosling Plague virus in Guangdong Province, and to provide data basis for the prevention and control of Gosling Plague virus in Guangdong Province.
【学位授予单位】：仲恺农业工程学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.33

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