新疆库蠓源性盖塔病毒的分离与鉴定

发布时间：2018-04-24 08:58

本文选题：新疆 + 库蠓　；参考：《畜牧兽医学报》2017年10期

【摘要】：为了解新疆尉犁县库蠓体内携带病毒情况,从该县采集18 000只库蠓,50只为一组研磨后分别接种于BHK-21、C6/36、Vero细胞,盲传5代,一组样品使BHK-21出现CPE,先用5-氟尿核苷药敏试验鉴定为RNA病毒,之后用虫媒RNA病毒引物扩增,鉴定为甲病毒,疑似盖塔病毒,最后用中和试验、免疫电镜观察和盖塔病毒C基因特异性引物PCR等方法鉴定分离毒株为盖塔病毒。本研究首次从新疆库蠓体内分离到盖塔病毒,该毒株与2005年日本分离株相似性高达98%,推测可能是由于引进日本带病种公畜或精液有关,提示在进出口畜牧贸易活动中,应加强盖塔病毒的检疫工作。
[Abstract]:In order to understand the virus transmission in Culicoides in Yuli County, Xinjiang, 18 000 Culicoides were collected from this county and inoculated into BHK-21 C6 / 36 Vero cells for 5 passages. A group of samples made BHK-21 appear RNA virus, first identified as RNA virus by 5-fluorouracil drug sensitivity test, then amplified with insect-mediated RNA virus primer, identified as A virus, suspected Gaeta virus, and finally used neutralization test. The isolated strain was identified as Gaeta virus by immunoelectron microscopy and specific primer PCR. In this study, Gaeta virus was first isolated from Culicoides kulicoides in Xinjiang. The similarity between the virus strain and the Japanese isolate in 2005 is as high as 98 kori, which suggests that the virus may be related to the introduction of Japanese diseased species, male animals or semen, suggesting that it is involved in the import and export of livestock trade. The quarantine of Gaeta virus should be strengthened.
【作者单位】：新疆畜牧科学院兽医研究所;新疆农业大学动物医学学院;新疆尉犁县畜牧兽医工作站;新疆巴音郭楞蒙古自治州动物疾病控制与诊断中心;
【基金】：公益性行业(农业)科研专项(201303035) 自治区公益性科研院所基本科研业务经费资助项目(KY2017002)
【分类号】：S852.65

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