SU6656对鸡传染性喉气管炎病毒与宿主细胞互作分子网络的影响

发布时间：2018-04-24 09:04

本文选题：传染性喉气管炎病毒 + RNA-seq　；参考：《中国预防兽医学报》2017年08期

【摘要】：为探索SU6656对鸡传染性喉气管炎病毒(ILTV)感染的影响及调控机制,本研究应用鸡LMH细胞系感染ILTV LJS09株作为实验模型,测定了SU6656对ILTV增殖和毒力的影响,通过高通量RNA测序技术(RNA-Seq)在全基因组范围内检测宿主细胞基因转录谱表达,并利用荧光定量PCR对部分差异表达基因进行验证。进一步的GO功能分析显示,在宿主细胞应答ILTV感染过程中,SU6656所调控基因主要涉及氢离子跨膜运输、辅酶Q的结合、Fe~(2+)和S~(2+)聚集等功能。KEGG通路分析表明,氧化磷酸化通路在SU6656调控ILTV感染的过程中可能发挥重要作用。本研究为进一步解析ILTV感染及致病机制提供了重要依据。
[Abstract]:In order to explore the effect of SU6656 on the infection of chicken infectious laryngotracheitis virus (ILTVV) and its regulatory mechanism, the effect of SU6656 on the proliferation and virulence of ILTV was determined by using chicken LMH cell line infected with ILTV LJS09 as the experimental model. High throughput RNA sequencing technique was used to detect the transcription profile of host cell genes in the whole genome, and some differentially expressed genes were verified by fluorescence quantitative PCR. Further go functional analysis showed that the genes regulated by SU6656 were mainly involved in the transmembrane transport of hydrogen ions, the binding of coenzyme Q to Fean2) and the aggregation of coenzyme Q). KEGG pathway analysis showed that, in the course of host cell response to ILTV infection, KEGG pathway analysis showed that Oxidative phosphorylation pathway may play an important role in the regulation of ILTV infection by SU6656. This study provides an important basis for further analysis of ILTV infection and pathogenesis.
【作者单位】：黑龙江八一农垦大学动物科技学院;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽呼吸道传染病创新团队;
【基金】：现代农业蛋鸡产业技术体系项目(CARS-41-K12) 国家科技支撑计划项目(2015BAD12B03)
【分类号】：S852.65

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