不同酶及组合处理对青贮水稻秸秆微观结构的影响

发布时间：2018-04-24 14:43

本文选题：秸秆 + 木质纤维素　；参考：《动物营养学报》2017年04期

【摘要】：本试验以水稻秸秆为研究对象,采用高效降解纤维素酶进行青贮发酵处理,分析其对水稻秸秆微观结构的影响,旨在实现农作物秸秆的高效降解,提高其营养价值。设青贮对照(S组)、复合酶制剂处理(C组)、果胶酶+漆酶处理(PL组)、复合酶制剂+果胶酶+漆酶处理(CPL组)和原料(M组),各组采用袋装真空青贮。室温贮藏45 d后取样,采用实验室检测方法、苯酚硫酸法、4,4'二羧酸-2,2'-喹啉(BCA)法、近红外光谱(FTIR)、X-射线衍射和伊红美兰法分析不同酶或酶组合处理的水稻秸秆青贮饲料的营养成分含量、发酵品质及微观结构变化。结果表明:1)与S组相比,PL组、C组及CPL组的乳酸(LA)含量显著增加(P0.05),p H、氨态氮/总氮及乙酸、纤维素含量均显著降低(P0.05);CPL组、C组的粗蛋白质含量显著降低(P0.05)。2)与S组相比,PL组青贮水稻秸秆的聚合度显著降低(P0.05),降低幅度达30.26%,分子间氢键结合力减弱,结晶度与比表面积无显著变化(P0.05);C组青贮水稻秸秆聚合度显著降低(P0.05),降低幅度达27.11%,分子间氢键结合力减弱,结晶度与比表面积无显著变化(P0.05);CPL组青贮水稻秸秆的聚合度显著降低(P0.05),降低幅度达56.32%,分子间氢键结合力减弱,有降低结晶度和增加比表面积的趋势(P0.05)。综上,CPL组降解纤维组分的效果最理想,能有效地破解细胞壁中木质素-纤维素-半纤维素复合结构,将纤维素降解为可利用的糖,提高水稻秸秆青贮饲料的营养含量,降低聚合度及结晶度,增大比表面积,从而提高秸秆的可消化性及利用率。
[Abstract]:In this experiment, rice straw was used as the research object, and the effect of cellulase degradation on the microstructure of rice straw was analyzed in order to achieve the high efficiency degradation of crop straw and improve its nutritional value. The silage control group S, the compound enzyme treatment group C, the Pectinase laccase group, the compound enzyme Pectinase laccase group (CPL group) and the raw material group M were treated with vacuum silage in each group. After storage at room temperature for 45 days, the samples were sampled by laboratory method, and the method of phenol sulfuric acid 4 'dicarboxylic acid-2O2O2-Quinoline (BCA) was used. The content of nutrients, fermentation quality and microstructure of rice straw silage treated with different enzyme or enzyme combination were analyzed by near infrared spectrum FTIR X-ray diffraction and ehongmeilan method. The results showed that the content of lactic acid in group C and CPL increased significantly than that in group S (P 0.05), ammonia nitrogen / total nitrogen and acetic acid. Compared with group S, the degree of polymerization of silage rice straw in Pl group was significantly lower than that in group S, the degree of polymerization of silage rice straw was decreased significantly, the reduction range was 30.26%, and the hydrogen bonding force between the molecules was decreased, compared with that of group S, the content of cellulose was significantly decreased, and the content of crude protein in group C was significantly decreased compared with that of group S. There was no significant change in crystallinity and specific surface area. The degree of polymerization of silage straw significantly decreased by 27.11%, and the intermolecular hydrogen bond strength decreased. There was no significant change in crystallinity and specific surface area. The degree of polymerization of silage rice straw in P0.05 / CPL group was significantly decreased by 56.32cm, and the hydrogen bonding force between molecules was decreased. There was a tendency of decreasing crystallinity and increasing specific surface area (P0.05N). The effect of CPL group on the degradation of fiber components is the best, which can effectively decipher the lignin-cellulose hemicellulose compound structure in the cell wall, degrade cellulose into available sugar, and improve the nutrient content of rice straw silage. By reducing the degree of polymerization and crystallinity and increasing the specific surface area, the digestibility and utilization ratio of straw can be improved.
【作者单位】：塔里木大学动物科学学院;中国农业科学院饲料研究所农业部饲料生物技术重点实验室;
【基金】：秸秆饲料生物转化技术研究与示范项目(20120304202) 国家肉羊产业技术体系建设专项资金(CARS-39)
【分类号】：S816

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