VCAN及GRAMD3基因多态性及其与鸡胫黑色素沉着的关系研究

发布时间：2018-04-25 00:15

本文选题：鸡 + 腿胫　；参考：《华中农业大学》2015年硕士论文

【摘要】：已有的研究推测VCAN 及 GRAMD3基因可能与黑色腿胫的形成有关,本研究的目的是研究Verican (VCAN) 和 Gram domain containing 3 (GRAMD3)基因及其与鸡胫色的关系。基于NCBI的数据,这两个基因分别位于五号染色体(Z染色体、即性染色体)长臂的14.3和23.2。本研究先利用DNA测序的方法在52只鸡的Versican 和 GRAMD3基因上分别寻找SNP,结果共找到12个突变位点,其中七个位于Versican、五个位于GRAMD3。分别对versican基因上的的内含子3(T161 C),内含子4(G 127 C),内含子5(C 143 T),外显子7(G 603 A),外显子s8(G 290A),外显子14(G 235 C),外显子15(C 154 T)以及GRAMD3基因上的外显子2(G 199T),内含子4(C 81 G),外显子6(G 111 C),内含子9(C 128 T)and内含子11(A84G)进行分型。通过PCR-RFLP对多态进行检测,并用Z检验进行分析,然后利用SPSS对检验结果进行显著性分析。在这两个基因中,我们鉴定出GRAMD3第四外显子的非编码区的单尾分析结果为显著的P0.028717。该突变为无义突变,因此没有改变编码蛋白质氨基酸序列的编码区组成。但我们推测该突变可能是为鸡胫色的连锁标记或者通过其他方式影响鸡的胫色。
[Abstract]:It has been speculated that VCAN and GRAMD3 genes may be related to the formation of black leg shank. The purpose of this study is to study the relationship between Verican and Gram domain containing 3 GRAMD3) genes and their relationship with chicken shank color. Based on NCBI data, the two genes are located at 14. 3 and 23. 2 of the long arm of chromosome 5, Z, or sex chromosome, respectively. In this study, DNA sequencing was used to search for Versican and GRAMD3 genes in 52 chickens respectively. The results showed that there were 12 mutation sites in which seven were located in Versica anand five in GRAMD3. The intron 3(T161 C, intron 4G127C, intron 5C143T, exon 7G 603A, exon s8G290A, exon 14G235CU, exon 15C154T) and GRAMD3 gene exon 2G199TU, intron 4G81 GG, exon 15C154T, exon 7G 603A, exon 8G 290A, exon 14G 235CU, exon 15C154T) and exon 2G199TN, intron 4C81G, exon 15C154T, respectively. 6G 111C, intron 9 C 128 T)and intron 11A 84 G) were classified. The polymorphism was detected by PCR-RFLP, analyzed by Z test, and the significance was analyzed by SPSS. Among the two genes, we identified the single tail analysis of the non-coding region of the fourth exon of GRAMD3 as a significant P0.028717. The mutation is nonsense, so it does not change the coding region composition of amino acid sequence of encoding protein. However, we speculate that the mutation may be a linkage marker of chicken shank color or influence the shank color in other ways.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831

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