番鸭CRHBP基因多态性及生物信息学分析

发布时间：2018-04-25 15:10

  本文选题：番鸭 + 异常行为　； 参考：《基因组学与应用生物学》2017年04期

【摘要】：为研究促肾上腺皮质激素释放激素结合蛋白(corticotropin-releasing hormone binding protein,CRHBP)作为番鸭异常行为研究候选基因的可能性。实验以半番鸭为实验材料,设计9对引物对CRHBP基因内含子和外显子序列进行扩增,其PCR产物经测序后,利用生物信息学软件分析SNPs位点突变前后CRHBP基因及其蛋白结构的变化。结果表明,在扩增的CRHBP基因中筛选出12个SNPs:Exon3-G~(5805)A、Exon3-C~(5906)T、Exon7-A~(10747)G、Intron3-A~(5984)G、Intron3-T~(6019)G、Intron3-C~(6021)T、Intron3-G~(6035)A、Intron3-G~(6036)A、Intron7-C~(10506)T、Intron7-A~(10613)G、Intron8-G~(12097)A和Intron8-G~(12118)A,其中Exon3-G~(5805)A和Exon7-A~(10747)G为同义突变;Exon3-C~(5906)T是错义突变,导致编码的缬氨酸(Val)突变为异亮氨酸(Ile);Intron3-A~(5984)G、Intron3-T~(6019)G、Intron3-C~(6021)T、Intron3-G~(6035)A、Intron3-G~(6036)A、Intron7-C~(10506)T、Intron7-A~(10613)G、Intron8-G~(12097)A和Intron8-G~(12118)A处于内含子区域,不参与氨基酸编码。
[Abstract]:In order to study the possibility of corticotropin-releasing hormone binding protein (CRHBP) as a candidate gene for the study of abnormal behavior in Muscovy ducks, the experiment was conducted with a semi muscovy duck as the experimental material. 9 pairs of primers were designed to amplify the intron and exons of the CRHBP gene, and the PCR products were sequenced. The changes of CRHBP gene and its protein structure before and after the mutation of SNPs site were analyzed by bioinformatics software. The results showed that 12 SNPs:Exon3-G~ (5805) A, Exon3-C~ (5906) T, Exon7-A~ (10747) G, Intron3-A~ (5984) G, 6019), 6036 (6036), 6036 (6036), were screened in the amplified CRHBP gene. 10506) T, Intron7-A~ (10613) G, Intron8-G~ (12097) A and Intron8-G~ (12118) A, in which Exon3-G~ (5805) A and Exon7-A~ (10747) G are synonymous mutations; Exon3-C~ (5906) is a missense mutation, resulting in the mutation of the encoded valine to isoleucine; 6019 (6021), 6035. Intron7-C~ (10506) T, Intron7-A~ (10613) G, Intron8-G~ (12097) A and Intron8-G~ (12118) A are in intron regions, and do not participate in amino acid coding.

【作者单位】： 贵州大学动物科学学院;高原山地动物遗传育种与繁殖省部共建教育部重点实验室;
【基金】：国家自然科学基金(31360566) 贵州省教育厅产学研基地项目(黔教合KY字2013-120号) 贵州省科技厅农业重大专项[黔科合重大专项字(2012)6004号]基金共同资助
【分类号】：Q811.4;S834
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本文编号：1801851