日粮中添加不同水平紫苏籽对湖羊生长性能、瘤胃发酵及养分表观消化率的影响

发布时间：2018-04-25 16:00

本文选题：湖羊 + 紫苏籽　；参考：《草业学报》2017年05期

【摘要】：本试验旨在研究日粮中添加不同水平紫苏籽(Perilla frutescens seed,PFS)对湖羊生长性能、血清生化指标、养分表观消化率及瘤胃发酵的影响。选取2月龄、平均初始体重为(23.02±1.36)kg的湖羊公羔60只,依据日粮中PFS含量随机分成为:对照组(Control)、5%紫苏籽组(5%PFS)、10%紫苏籽组(10%PFS)和15%紫苏籽组(15%PFS)。预试期14d,正试期70d。饲喂试验结束前21d,从各组随机挑选4只羊进行7d的消化代谢试验。试验结束后称重,计算平均日增重(ADG),并采集瘤胃液和血液分别用气相色谱法、比色法、全自动生化分析仪和酶联免疫分析法测定瘤胃液挥发性脂肪酸(VFA)、氨态氮(NH_3-N)、血清中总蛋白(TP)、尿素氮(UN)、葡萄糖(Glu)和血清中生长激素(GH)、胰岛素样生长因子-1(IGF-1)等。结果表明,日粮中PFS水平对湖羊生长性能(干物质采食量、平均日增重和料重比)、血清生化指标(TP、UN、Glu、GH和IGF-1)、瘤胃pH和瘤胃液乙酸、丙酸及总挥发性脂肪酸(TVFA)等含量的影响不显著(P0.05),但随着PFS添加水平的升高,乙酸、丁酸和TVFA的含量呈降低趋势,丙酸呈上升趋势;与对照组相比,15%PFS组中NH_3-N和乙酸/丙酸极显著降低(P0.01);添加PFS不影响日粮中粗脂肪(EE)和酸性洗涤纤维(ADF)的表观消化率(P0.05),但干物质(DM)、有机物(OM)和中性洗涤纤维(NDF)的表观消化率随着PFS添加水平升高而下降,且15%PFS组的DM和OM的表观消化率均极显著低于对照组(P0.01);与对照组相比,10%PFS组的粗蛋白(CP)表观消化率极显著提高(P0.01),而15%PFS组的极显著降低(P0.01)。综上所述,湖羊日粮中添加PFS水平为10%时,饲喂效果较好。
[Abstract]:The purpose of this study was to study the effects of different levels of perilla frutescens Seedling on growth performance, serum biochemical index, apparent nutrient digestibility and rumen fermentation of Hu sheep. A total of 60 male lambs with average initial body weight of 23.02 卤1.36)kg (2 months old) were randomly divided into two groups according to their dietary PFS content: the control group with 5% perilla seed group and the control group with 10% perilla seed group and 15% perilla seed group. The pre-test period was 14 days and the positive test period was 70 days. Four sheep were randomly selected from each group 21 days before the end of feeding test for 7 days of digestion and metabolism test. At the end of the experiment, the average daily gain was calculated, and the rumen fluid and blood were collected by gas chromatography and colorimetry, respectively. Automatic biochemical analyzer and enzyme-linked immunosorbent assay (Elisa) were used to determine the levels of volatile fatty acids (VFAA) in rumen fluid (VFAA), ammonia nitrogen (NH3-N), total protein (TPN) in serum, urea nitrogen (UN), glucosamine (Glu), growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in serum. The results showed that the effects of dietary PFS level on growth performance (dry matter intake, average daily gain and feed weight ratio), serum biochemical indexes, such as GH and IGF-1, rumen pH and rumen liquid acetic acid were studied. The contents of propionic acid and total volatile fatty acid (TVB) were not significantly affected by P0.05, but with the increase of PFS, the contents of acetic acid, butyric acid and TVFA decreased and propionic acid increased. Compared with the control group, the apparent digestibility of NH_3-N and acetic acid / propionic acid in PFS group was significantly lower than that in the control group, and the apparent digestibility of dietary crude fat and acid detergent fiber was not affected by adding PFS, but the apparent digestibility of dry matter (DMN), organic matter (Om) and neutral detergent fiber (NDF) were not affected by adding PFS. The digestibility decreased with the increase of PFS supplementation level. The apparent digestibility of DM and OM in the 15%PFS group was significantly lower than that in the control group (P 0.01), and the apparent digestibility of CP in the 10 PFS group was significantly higher than that in the control group, while that in the 15%PFS group was significantly lower than that in the control group. To sum up, the feed effect of Hu sheep was better when the PFS level was 10%.
【作者单位】：南京农业大学江苏省肉羊产业工程技术研究中心;江苏省泰州市海伦羊业有限公司;
【基金】：公益性行业(农业)科研专项,南方地区肉羊育肥与高品质肉生产技术研究(201303144) 国家现代肉羊产业技术体系项目(nycytx-39)资助
【分类号】：S826.5

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