山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析

发布时间：2018-04-26 03:02

本文选题：山羊副流感病毒型 + miRNA　；参考：《畜牧兽医学报》2017年05期

【摘要】：本研究旨在分析山羊副流感病毒3型(CPIV3)感染MDBK细胞和正常MDBK细胞差异表达的miRNA,以探索miRNA在CPIV3感染过程中的作用及其调控机制。将CPIV3感染MDBK细胞,利用高通量测序技术检测分析MDBK细胞中miRNA表达谱,并筛选差异表达的miRNA。对靶基因进行GO注释和KEGG富集。结果显示,有37个miRNA显著差异表达(P0.001),其中18个miRNA在病毒感染中上调,19个miRNA在病毒感染中下调。经RT-qPCR方法验证5个差异表达的候选miRNA,结果与高通量测序分析有很好的一致性。进一步GO功能注释表明:差异表达的miRNA靶基因生物学功能相对集中在免疫调节、细胞生物调控及细胞新陈代谢等生物过程。这些靶基因参与多种细胞的信号通路,包括细胞黏附分子、B细胞受体信号通路、MAPK信号通路、代谢通路、黏合斑、钙离子信号通路和趋化因子信号通路等。本研究为进一步探讨CPIV3致病机制奠定了基础。
[Abstract]:The purpose of this study was to analyze the differential expression of miRNAs in MDBK cells and normal MDBK cells infected by goat parainfluenza virus type 3 (CPIV3) in order to explore the role of miRNA in the process of CPIV3 infection and its regulatory mechanism. High throughput sequencing technique was used to detect and analyze the miRNA expression profile of MDBK cells infected with CPIV3 and to screen differentially expressed miRNAs. The target gene was annotated by go and enriched with KEGG. The results showed that there were 37 miRNA differentially expressed p0.001, of which 18 miRNA were up-regulated and 19 miRNA down-regulated in viral infection. Five differentially expressed candidate miRNAs were verified by RT-qPCR method, and the results were in good agreement with high throughput sequencing analysis. Further go functional annotations showed that the biological functions of differentially expressed miRNA target genes were relatively concentrated in biological processes such as immunomodulation, cellular biological regulation and cell metabolism. These target genes are involved in many cell signaling pathways, including cell adhesion molecule B cell receptor signaling pathway, MAPK signal pathway, metabolic pathway, adhesion plaque, calcium signal pathway and chemokine signaling pathway. This study laid a foundation for further study of the pathogenesis of CPIV3.
【作者单位】：江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室;
【基金】：江苏省农业科技自主创新资金项目[CX(15)1061] 国家自然科学基金(31402180) 十三五国家重点研发计划项目(2016YFD0500908)
【分类号】：S852.654

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