两种进口佐剂对绵羊肺炎支原体灭活疫苗免疫小鼠抗体水平影响的研究

发布时间：2018-04-26 16:18

本文选题：绵羊肺炎支原体 + 灭活疫苗　；参考：《黑龙江畜牧兽医》2016年23期

【摘要】：为了筛选绵羊肺炎支原体(MO)灭活疫苗的佐剂,试验采用绵羊肺炎支原体新疆分离株扩大培养后得到灭活抗原,分别与两种不同佐剂ISA 201和ISA 206混合乳化制备灭活疫苗,再以不同剂量免疫健康成年小鼠,最后用MO间接血凝的方法分别测定二免后第7,14,21,28天血清抗体效价。结果表明:ISA 206佐剂疫苗0.3 m L剂量组在第14天抗体效价就达到较高水平,第21天后开始下降;ISA 206佐剂疫苗0.2 m L剂量组在二免后第14天抗体效价达到高峰(1∶128),且在第21天仍维持抗体高峰,第28天开始下降;ISA 201佐剂疫苗0.3 m L剂量组在二免疫后第14天抗体效价达到高峰,维持1周后开始下降;ISA 201佐剂疫苗0.2 m L组免疫应答较慢,在二免后第21天才达到最高抗体效价(1∶64)。说明ISA 206佐剂诱导产生MO抗体较快,维持时间长,优于ISA 201佐剂,可作为MO灭活疫苗的优选佐剂。
[Abstract]:In order to screen the adjuvant of the inactivated vaccine of Mycoplasma pneumoniae in sheep, the inactivated antigen was obtained by using the Xinjiang isolate of Mycoplasma pneumoniae in the experiment. The inactivated vaccine was prepared by emulsifying with two different adjuvants, ISA 201 and ISA 206, respectively. Then the healthy adult mice were immunized with different doses, and the titer of serum antibody was determined by MO indirect hemagglutination method on the 28th day after the second immunization. The results showed that the antibody titer of the 0.3 mL dose group reached a higher level on the 14th day. After 21 days, the antibody titers of the 0.2 mL dose group of the adjuvant vaccine of ISA206 reached a peak of 1: 128 on the 14th day after the second immunization, and the antibody peak was maintained on the 21st day. On the 28th day, the antibody titers reached the peak on the 14th day after the second immunization in the 0.3 mL dose group of SASA201 adjuvant vaccine, and the immune response was slower in 0.2 mL group of 0.2 mL adjuvant vaccine of ISA 201 vaccine after one week of maintenance. The highest antibody titer was 1: 64 on the 21st day after the second immunization. The results showed that ISA 206 adjuvant could be used as an adjuvant for MO inactivated vaccine because of its rapid production of MO antibody and long maintenance time, which was superior to that of ISA 201 adjuvant.
【作者单位】：石河子大学动物科技学院;
【基金】：兵团重大科技项目(2013AA003-3)
【分类号】：S852.4

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